6kw4: Difference between revisions

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-'''Unreleased structure'''
-The entry 6kw4 is ON HOLD
+==The ClassB RSC-Nucleosome Complex==
+<SX load='6kw4' size='340' side='right' viewer='molstar' caption='[[6kw4]], [[Resolution|resolution]] 7.55&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6kw4]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KW4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.55&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kw4 OCA], [https://pdbe.org/6kw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kw4 RCSB], [https://www.ebi.ac.uk/pdbsum/6kw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kw4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/H32_XENLA H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The RSC complex remodels chromatin structure and regulates gene transcription. We report the cryoEM structure of yeast RSC bound to the nucleosome. RSC is delineated into the ATPase motor, the actin-related-protein (ARP) module, and the substrate-recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Together, our findings shed light on RSC assembly and functionality, and provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers.
-Authors:
+Structure of the RSC complex bound to the nucleosome.,Ye Y, Wu H, Chen K, Clapier CR, Verma N, Zhang W, Deng H, Cairns BR, Gao N, Chen Z Science. 2019 Oct 31. pii: science.aay0033. doi: 10.1126/science.aay0033. PMID:31672915<ref>PMID:31672915</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6kw4" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
+*[[Helicase 3D structures|Helicase 3D structures]]
+*[[Histone 3D structures|Histone 3D structures]]
+== References ==
+<references/>
+__TOC__
+</SX>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Saccharomyces cerevisiae S288C]]
+[[Category: Xenopus laevis]]
+[[Category: Cairns B]]
+[[Category: Chen KJ]]
+[[Category: Chen ZC]]
+[[Category: Gao N]]
+[[Category: Verma N]]
+[[Category: Wu H]]
+[[Category: Ye YP]]