3am7: Difference between revisions

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-[[Image:3am7.jpg|left|200px]]
-<!--
+==Crystal structure of the ternary complex of eIF4E-M7GTP-4EBP2 peptide==
-The line below this paragraph, containing "STRUCTURE_3am7", creates the "Structure Box" on the page.
+<StructureSection load='3am7' size='340' side='right'caption='[[3am7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3am7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AM7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AM7 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene></td></tr>
-{{STRUCTURE_3am7|  PDB=3am7  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3am7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3am7 OCA], [https://pdbe.org/3am7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3am7 RCSB], [https://www.ebi.ac.uk/pdbsum/3am7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3am7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+To clarify the higher eukaryotic initiation factor 4E (eIF4E) binding selectivity of 4E-binding protein 2 (4E-BP2) than of 4E-BP1, as determined by Trp fluorescence analysis, the crystal structure of the eIF4E binding region of 4E-BP2 in complex with m(7) GTP-bound human eIF4E has been determined by X-ray diffraction analysis and compared with that of 4E-BP1. The crystal structure revealed that the Pro47-Ser65 moiety of 4E-BP2 adopts a L-shaped conformation involving extended and alpha-helical structures and extends over the N-terminal loop and two different helix regions of eIF4E through hydrogen bonds, and electrostatic and hydrophobic interactions; these features were similarly observed for 4E-BP1. Although the pattern of the overall interaction of 4E-BP2 with eIF4E was similar to that of 4E-BP1, a notable difference was observed for the 60-63 sequence in relation to the conformation and binding selectivity of the 4E-BP isoform, i.e. Met-Glu-Cys-Arg for 4E-BP1 and Leu-Asp-Arg-Arg for 4E-BP2. In this paper, we report that the structural scaffold of the eIF4E binding preference for 4E-BP2 over 4E-BP1 is based on the stacking of the Arg63 planar side chain on the Trp73 indole ring of eIF4E and the construction of a compact hydrophobic space around the Trp73 indole ring by the Leu59-Leu60 sequence of 4E-BP2.
-===Crystal structure of the ternary complex of eIF4E-M7GTP-4EBP2 peptide===
+Structural scaffold for eIF4E binding selectivity of 4E-BP isoforms: crystal structure of eIF4E binding region of 4E-BP2 and its comparison with that of 4E-BP1.,Fukuyo A, In Y, Ishida T, Tomoo K J Pept Sci. 2011 Sep;17(9):650-7. doi: 10.1002/psc.1384. Epub 2011 Jun 10. PMID:21661078<ref>PMID:21661078</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3am7" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[3am7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AM7 OCA].
+*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Fukuyo, A.]]
+[[Category: Large Structures]]
-[[Category: In, Y.]]
+[[Category: Fukuyo A]]
-[[Category: Ishida, T.]]
+[[Category: In Y]]
-[[Category: Tomoo, K.]]
+[[Category: Ishida T]]
-[[Category: Cap]]
+[[Category: Tomoo K]]
-[[Category: Protein-protein complex]]
-[[Category: Translation]]
-[[Category: Translation-protein binding complex]]