2kma: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2kma.png|left|200px]]
-<!--
+==NMR structure of the F0F1 double domain (residues 1-202) of the talin ferm domain==
-The line below this paragraph, containing "STRUCTURE_2kma", creates the "Structure Box" on the page.
+<StructureSection load='2kma' size='340' side='right'caption='[[2kma]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2kma]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KMA FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kma OCA], [https://pdbe.org/2kma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kma RCSB], [https://www.ebi.ac.uk/pdbsum/2kma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kma ProSAT]</span></td></tr>
-{{STRUCTURE_2kma|  PDB=2kma  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/km/2kma_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kma ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Talin is a 270-kDa protein that activates integrins and couples them to cytoskeletal actin. Talin contains an N-terminal FERM domain comprised of F1, F2 and F3 domains, but it is atypical in that F1 contains a large insert and is preceded by an extra domain F0. Although F3 contains the binding site for beta-integrin tails, F0 and F1 are also required for activation of beta1-integrins. Here, we report the solution structures of F0, F1 and of the F0F1 double domain. Both F0 and F1 have ubiquitin-like folds joined in a novel fixed orientation by an extensive charged interface. The F1 insert forms a loop with helical propensity, and basic residues predicted to reside on one surface of the helix are required for binding to acidic phospholipids and for talin-mediated activation of beta1-integrins. This and the fact that basic residues on F2 and F3 are also essential for integrin activation suggest that extensive interactions between the talin FERM domain and acidic membrane phospholipids are required to orientate the FERM domain such that it can activate integrins.
-===NMR structure of the F0F1 double domain (residues 1-202) of the talin ferm domain===
+Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation.,Goult BT, Bouaouina M, Elliott PR, Bate N, Patel B, Gingras AR, Grossmann JG, Roberts GC, Calderwood DA, Critchley DR, Barsukov IL EMBO J. 2010 Mar 17;29(6):1069-80. Epub 2010 Feb 11. PMID:20150896<ref>PMID:20150896</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_20150896}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2kma" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 20150896 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_20150896}}
-==About this Structure==
-[[2kma]] is a 1 chain structure of [[Talin]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KMA OCA].
 ==See Also==
-*[[Talin]]
+*[[Talin 3D structures|Talin 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:20150896</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Barsukov, I L.]]
+[[Category: Barsukov IL]]
-[[Category: Bate, N.]]
+[[Category: Bate N]]
-[[Category: Critchley, D R.]]
+[[Category: Critchley DR]]
-[[Category: Elliott, P R.]]
+[[Category: Elliott PR]]
-[[Category: Goult, B T.]]
+[[Category: Goult BT]]
-[[Category: Roberts, G C.]]
+[[Category: Roberts GC]]
-[[Category: Adhesion]]
-[[Category: Cytoplasm]]
-[[Category: Cytoskeleton]]
-[[Category: F0]]
-[[Category: F1]]
-[[Category: Ferm]]
-[[Category: Membrane]]
-[[Category: N-terminal]]
-[[Category: Structural protein]]
-[[Category: Talin]]