2kby: Difference between revisions

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-[[Image:2kby.png|left|200px]]
-{{STRUCTURE_2kby|  PDB=2kby  |  SCENE=  }}
+==The Tetramerization Domain of Human p73==
+<StructureSection load='2kby' size='340' side='right'caption='[[2kby]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2kby]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KBY FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kby OCA], [https://pdbe.org/2kby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kby RCSB], [https://www.ebi.ac.uk/pdbsum/2kby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kby ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/P73_HUMAN P73_HUMAN] Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.<ref>PMID:11753569</ref> <ref>PMID:10203277</ref> <ref>PMID:18174154</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kby_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kby ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+p73 and p63, the two ancestral members of the p53 family, are involved in neurogenesis, epithelial stem cell maintenance and quality control of female germ cells. The highly conserved oligomerization domain (OD) of tumor suppressor p53 is essential for its biological functions, and its structure was believed to be the prototype for all three proteins. However, we report that the ODs of p73 and p63 differ from the OD of p53 by containing an additional alpha-helix that is not present in the structure of the p53 OD. Deletion of this helix causes a dissociation of the OD into dimers; it also causes conformational instability and reduces the transcriptional activity of p73. Moreover, we show that ODs of p73 and p63 strongly interact and that a large number of different heterotetramers are supported by the additional helix. Detailed analysis shows that the heterotetramer consisting of two homodimers is thermodynamically more stable than the two homotetramers. No heterooligomerization between p53 and the p73/p63 subfamily was observed, supporting the notion of functional orthogonality within the p53 family.
-===The Tetramerization Domain of Human p73===
+Conformational stability and activity of p73 require a second helix in the tetramerization domain.,Coutandin D, Lohr F, Niesen FH, Ikeya T, Weber TA, Schafer B, Zielonka EM, Bullock AN, Yang A, Guntert P, Knapp S, McKeon F, Ou HD, Dotsch V Cell Death Differ. 2009 Dec;16(12):1582-9. Epub 2009 Sep 18. PMID:19763140<ref>PMID:19763140</ref>
-{{ABSTRACT_PUBMED_19763140}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 2kby" style="background-color:#fffaf0;"></div>
-[[2kby]] is a 4 chain structure of [[P73]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBY OCA].
 ==See Also==
 *[[P73|P73]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019763140</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Coutandin, D.]]
+[[Category: Large Structures]]
-[[Category: Doetsch, V.]]
+[[Category: Coutandin D]]
-[[Category: Guntert, P.]]
+[[Category: Doetsch V]]
-[[Category: Ikeya, T.]]
+[[Category: Guntert P]]
-[[Category: Loehr, F.]]
+[[Category: Ikeya T]]
-[[Category: Ou, H D.]]
+[[Category: Loehr F]]
-[[Category: Activator]]
+[[Category: Ou HD]]
-[[Category: Anti-oncogene]]
-[[Category: Apoptosis]]
-[[Category: Cell cycle]]
-[[Category: Dna-binding]]
-[[Category: Metal-binding]]
-[[Category: Nucleus]]
-[[Category: Phosphoprotein]]
-[[Category: Tetramerization domain]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]