2jv3: Difference between revisions

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-[[Image:2jv3.gif|left|200px]]<br /><applet load="2jv3" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2jv3" />
-'''Ets-1 PNT domain (29-138) NMR structure ensemble'''<br />
-==Overview==
+==Ets-1 PNT domain (29-138) NMR structure ensemble==
-The Pointed (PNT) domain and an adjacent mitogen-activated protein (MAP) kinase phosphorylation site are defined by sequence conservation among a subset of ets transcription factors and are implicated in two regulatory strategies, protein interactions and posttranslational modifications, respectively. By using NMR, we have determined the structure of a 110-residue fragment of murine Ets-1 that includes the PNT domain and MAP kinase site. The Ets-1 PNT domain forms a monomeric five-helix bundle. The architecture is distinct from that of any known DNA- or protein-binding module, including the helix-loop-helix fold proposed for the PNT domain of the ets protein TEL. The MAP kinase site is in a highly flexible region of both the unphosphorylated and phosphorylated forms of the Ets-1 fragment. Phosphorylation alters neither the structure nor monomeric state of the PNT domain. These results suggest that the Ets-1 PNT domain functions in heterotypic protein interactions and support the possibility that target recognition is coupled to structuring of the MAP kinase site.
+<StructureSection load='2jv3' size='340' side='right'caption='[[2jv3]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2jv3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bqv 1bqv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JV3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jv3 OCA], [https://pdbe.org/2jv3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jv3 RCSB], [https://www.ebi.ac.uk/pdbsum/2jv3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jv3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ETS1_MOUSE ETS1_MOUSE] Transcription factor.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jv/2jv3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jv3 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-JV3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry 1BQV. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV3 OCA].
+*[[Ets1|Ets1]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure of the Ets-1 pointed domain and mitogen-activated protein kinase phosphorylation site., Slupsky CM, Gentile LN, Donaldson LW, Mackereth CD, Seidel JJ, Graves BJ, McIntosh LP, Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12129-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9770451 9770451]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Single protein]]
+[[Category: Kang H]]
-[[Category: Kang, H.]]
+[[Category: Lawrence MP]]
-[[Category: Lawrence, M P.]]
+[[Category: Lee GM]]
-[[Category: Lee, G M.]]
+[[Category: Schaerpf M]]
-[[Category: Schaerpf, M.]]
+[[Category: Slupsky CM]]
-[[Category: Slupsky, C M.]]
-[[Category: alpha-helical bundle]]
-[[Category: dna-binding]]
-[[Category: ets-1 pointed (pnt) domain]]
-[[Category: map kinase phosphorylation site]]
-[[Category: nucleus]]
-[[Category: transcription factor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:06:08 2008''