2hw0: Difference between revisions
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==NMR Solution Structure of the nuclease domain from the Replicator Initiator Protein from porcine circovirus PCV2== | ==NMR Solution Structure of the nuclease domain from the Replicator Initiator Protein from porcine circovirus PCV2== | ||
<StructureSection load='2hw0' size='340' side='right'caption='[[2hw0 | <StructureSection load='2hw0' size='340' side='right'caption='[[2hw0]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2hw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2hw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_circovirus_2 Porcine circovirus 2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HW0 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw0 OCA], [https://pdbe.org/2hw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2hw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hw0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw0 OCA], [https://pdbe.org/2hw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2hw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hw0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/REP_PCV2 REP_PCV2] Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep and/or Rep' binds a specific hairpin at the genome origin of replication. Introduces an endonucleolytic nick within the conserved sequence 5'-AGTATTAC-3' in the intergenic region of the genome, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, and nucleotidyl transferase.<ref>PMID:12954212</ref> <ref>PMID:25768890</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Porcine circovirus 2]] | ||
[[Category: Byeon | [[Category: Byeon IL]] | ||
[[Category: Campos-Olivas | [[Category: Campos-Olivas R]] | ||
[[Category: Gronenborn | [[Category: Gronenborn AM]] | ||
[[Category: Gronenborn | [[Category: Gronenborn B]] | ||
[[Category: Vega-Rocha | [[Category: Vega-Rocha S]] | ||