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New page: left|200px<br /><applet load="2g7h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g7h" /> '''Structure of an O6-Methylguanine DNA Methylt... |
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== | ==Structure of an O6-Methylguanine DNA Methyltransferase from Methanococcus jannaschii (MJ1529)== | ||
The structure of an O6-methylguanine-DNA methyltransferase (MGMT) from the | <StructureSection load='2g7h' size='340' side='right'caption='[[2g7h]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2g7h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G7H FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g7h OCA], [https://pdbe.org/2g7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g7h RCSB], [https://www.ebi.ac.uk/pdbsum/2g7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g7h ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/OGT_METJA OGT_METJA] Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.<ref>PMID:16826543</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/2g7h_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g7h ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of an O6-methylguanine-DNA methyltransferase (MGMT) from the thermophile Methanococcus jannaschii has been determined using multinuclear multidimensional NMR spectroscopy. The structure is similar to homologs from other organisms that have been determined by crystallography, with some variation in the N-terminal domain. The C-terminal domain is more highly conserved in both sequence and structure. Regions of the protein show broadening, reflecting conformational flexibility that is likely related to function. | |||
Structural studies of MJ1529, an O6-methylguanine-DNA methyltransferase.,Roberts A, Pelton JG, Wemmer DE Magn Reson Chem. 2006 Jul;44 Spec No:S71-82. PMID:16826543<ref>PMID:16826543</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
<div class="pdbe-citations 2g7h" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Roberts A]] | |||
[[Category: Roberts | |||
Latest revision as of 21:58, 29 May 2024
Structure of an O6-Methylguanine DNA Methyltransferase from Methanococcus jannaschii (MJ1529)Structure of an O6-Methylguanine DNA Methyltransferase from Methanococcus jannaschii (MJ1529)
Structural highlights
FunctionOGT_METJA Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe structure of an O6-methylguanine-DNA methyltransferase (MGMT) from the thermophile Methanococcus jannaschii has been determined using multinuclear multidimensional NMR spectroscopy. The structure is similar to homologs from other organisms that have been determined by crystallography, with some variation in the N-terminal domain. The C-terminal domain is more highly conserved in both sequence and structure. Regions of the protein show broadening, reflecting conformational flexibility that is likely related to function. Structural studies of MJ1529, an O6-methylguanine-DNA methyltransferase.,Roberts A, Pelton JG, Wemmer DE Magn Reson Chem. 2006 Jul;44 Spec No:S71-82. PMID:16826543[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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