2e5g: Difference between revisions
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== | ==Solution structure of RNA binding domain in RNA binding motif protein 21== | ||
<StructureSection load='2e5g' size='340' side='right'caption='[[2e5g]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2e5g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E5G FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e5g OCA], [https://pdbe.org/2e5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e5g RCSB], [https://www.ebi.ac.uk/pdbsum/2e5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e5g ProSAT], [https://www.topsan.org/Proteins/RSGI/2e5g TOPSAN]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/STPAP_HUMAN STPAP_HUMAN] Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.<ref>PMID:16790842</ref> <ref>PMID:18288197</ref> <ref>PMID:21102410</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/2e5g_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e5g ConSurf]. | |||
<div style="clear:both"></div> | |||
==See Also== | |||
*[[RNA uridylyltransferase|RNA uridylyltransferase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Inoue M]] | |||
[[Category: Inoue | [[Category: Kigawa T]] | ||
[[Category: Kigawa | [[Category: Muto Y]] | ||
[[Category: Muto | [[Category: Shirouzu M]] | ||
[[Category: Terada T]] | |||
[[Category: Shirouzu | [[Category: Tsuda K]] | ||
[[Category: Terada | [[Category: Yokoyama S]] | ||
[[Category: Tsuda | |||
[[Category: Yokoyama | |||