Argonaute: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

João Rodrigues, Michal Harel, Joel L. Sussman, Alexander Berchansky

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+<StructureSection load='4ola' size='350' side='right' scene='51/512703/Cv/1' caption='X-ray structure of the entire human Argonaute2 protein complex with RNA (red) (PDB [[4ola]]) at 2.3Å resolution'>
+==Function==
 The '''Argonaute''' protein is a component of the RISC complex, central to the [[RNA_Interference|RNA-induced silencing]] in eukaryotic organisms <ref name='ref1'>pmid 22539551</ref>. It is found in all higher eukaryotes and it plays an important role in a variety of processes as diverse as embryonic development, cell diferentiation and transposon silencing. These proteins are evolutionarily conserved and can be divided in three subfamilies: Ago, Piwi and Wago. The first are ubiquitously expressed and interact with siRNAs or miRNAs to participate in post-transcriptional gene silencing, both by destabilizing mRNA or by repressing the translation event. Piwi proteins are generally restricted to the germ line and associate piRNAs to mediate silencing of mobile genetic elements <ref name='ref2'>pmid 18304383</ref>. The third and final subclass, Wago, are worm specific.  For more details see [[RNA Interference]].
+*'''Argonaute 2''' is called '''slicer''' and is required for the degradation of MicroRNA molecules<ref>pmid 24263100</ref>.
+*'''Argonaute 4''' is involved in human RNA-dependent DNA methylation<ref>pmid 31333722</ref>.
+*'''Argonaute 10''' is involved in the degradation of MicroRNA molecules<ref>pmid 33571183</ref>.
 ==Structural Organisation==
-<Structure load='4EI1' size='350' frame='true' align='right' caption='X-ray structure of the entire human Argonaute2 protein (PDB [[4EI1]]) at 2.3Å resolution ' scene='Argonaute/Initial/1'/>
-There are two main structural features common to all Argonaute proteins: the [[PAZ|Paz domain]] and the [[PIWI|PIWI domain]]. Other structural features include the N domain and the Mid domain.
+There are two main structural features common to all Argonaute proteins: the Paz domain and the PIWI domain. Other structural features include the N domain and the Mid domain.
 ===Paz Domain===
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 == 3D Structures of argonaute ==
+[[Argonaute 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-===Argonaute 1===
-[[1r4k]] – DmAGO1 PAZ domain – ''Drosophila melanogaster''<br />
-[[1si2]], [[1si3]] – hAGO1 PAZ domain + RNA - human<br />
-[[4g0p]], [[4g0x]], [[3vna]] – AtAGO1 MID domain – ''Arabidopsis thaliana''<br />
-[[3vnb]] – AtAGO2 MID domain (mutant)<br />
-[[4g0q]], [[4g0y]], [[4g0z]] – AtAGO2 MID domain + nucleotide<br />
-===Argonaute 2===
-[[1r6z]] – DmAGO2 PAZ domain/MBP <br />
-[[1vyn]] – DmAGO2 PAZ domain - NMR <br />
-[[1t2r]], [[1t2s]] – DmAGO2 PAZ domain + RNA - NMR<br />
-[[3mj0]] – DmAGO2 PAZ domain + RNA <br />
-[[3luc]], [[3luk]] – hAGO2 MID domain <br />
-[[3lud]], [[3lug]], [[3luh]], [[3luj]], [[3qx8]], [[3qx9]] – hAGO2 MID domain + nucleotide<br />
-[[4ei1]], [[4ei3]] – hAGO2 (mutant) + RNA <br />
-[[4f3t]] – hAGO2 + RNA <br />
-[[4g0m]], [[4g0o]] – AtAGO2 MID domain <br />
-===Argonaute===
-[[1u04]], [[1z25]], [[1z26]] – AGO (mutant) – ''Pyrococcus furiosus'' <br />
-[[2f8s]], [[2f8t]] – AaAGO + RNA – ''Aquifex aeolicus''<br />
-[[2f8t]] – AaAGO <br />
-[[3da5]] – AGO  – ''Thermococcus thioreducens''<br />
-[[3dlb]], [[3dlh]], [[3f73]], [[3hm9]], [[3hvr]], [[3hxm]] – TtAGO + DNA – ''Thermus thermophilus''<br />
-[[3hjf]], [[3hk2]], [[3ho1]] – TtAGO (mutant) + DNA <br />
 ==External Resources==
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 ==References==
 <references/>
+[[Category:Topic Page]]