Arabinanase: Difference between revisions

Latest revision as of 12:03, 27 May 2024

Function

Arabinanase (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans.^[1] See more details in Exo-alpha-1,5-L-Arabinanase. Endo-arabinanase hydrolyzes from the N-terminal of the chain while exo-arabinanase hydrolyzes from the end of the chain.

Structural highlights

The .^[2]. .

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3D Structures of Arabinanase

Arabinanase 3D structures

Endo-arabinanase complex with arabinohexose (yellow) and Cl- ion (green) (PDB code 1gye)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G, Shoham Y, Shoham G. Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrate. Biochem J. 2009 Jul 29;422(1):73-82. PMID:19505290 doi:10.1042/BJ20090180
↑ Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ. Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486 doi:10.1038/nsb835

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Michal Harel, Alexander Berchansky

[1] Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G, Shoham Y, Shoham G. Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrate. Biochem J. 2009 Jul 29;422(1):73-82. PMID:19505290 doi:10.1042/BJ20090180

[2] Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ. Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486 doi:10.1038/nsb835

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 == Function ==
-'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].
+'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].  '''Endo-arabinanase''' hydrolyzes from the N-terminal of the chain while '''exo-arabinanase''' hydrolyzes from the end of the chain.
 == Structural highlights ==
 The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>.
+*<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>.
 *<scene name='45/451702/Cv/6'>Cl coordination site</scene>.
-*<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>.
-</StructureSection>
 == 3D Structures of Arabinanase ==
+[[Arabinanase 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*Endo-arabinanase
-**[[2x8f]], [[1uv4]] – BsEndo-Ara – ''Bacillus subtilis''<br />
-**[[2x8t]], [[4cot]] - BsEndo-Ara (mutant) <br />
-**[[3cu9]], [[1wl7]], [[5ho0]], [[5ho2]], [[5hp6]] – GsEndo-Ara – ''Geobacillus stearothermophilus''<br />
-**[[3d5y]], [[3d60]] - GsEndo-Ara (mutant) <br />
-**[[1gyd]] – CcEndo-Ara – ''Cellvibrio cellulose''<br />
-**[[1gyh]] - CcEndo-Ara (mutant)<br />
-**[[4kc7]], [[4kc8]] - TpEndo-Ara – ''Thermotoga petrophila''<br />
-**[[4kca]], [[4kcb]] - Endo-Ara – bovine<br />
-*Endo-arabinanase complex with substrate
-**[[1gye]] - CcEndo-Ara (mutant) + arabinohexose<br />
-**[[3d5z]], [[3d61]] - GsEndo-Ara (mutant) + arabinotriose<br />
-**[[5ho9]] - GsEndo-Ara + arabinooctaose<br />
-**[[5hof]] - GsEndo-Ara + pentaose<br />
-**[[5hon]] - GsEndo-Ara + arabinotriose<br />
-**[[2x8s]] - BsEndo-Ara (mutant) + arabinotriose<br />
-*Exo-arabinanase
-**[[3a71]] – PcExo-Ara residues 24-378 – ''Penicillium chrysogenum''<br />
-**[[2w5n]] – GzExo-Ara – ''Gibberella zeae''
-*Exo-arabinanase complex with substrate
-**[[3a72]] - PcExo-Ara residues 24-378 + arabinobiose<br />
-**[[2w5o]] - GzExo-Ara + arabinobiose<br />
-}}
 == References ==