Arabinanase: Difference between revisions

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== Function ==
== Function ==


'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].
'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].  '''Endo-arabinanase''' hydrolyzes from the N-terminal of the chain while '''exo-arabinanase''' hydrolyzes from the end of the chain.


== Structural highlights ==
== Structural highlights ==


The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>
The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>.
 
*<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>.
</StructureSection>
*<scene name='45/451702/Cv/6'>Cl coordination site</scene>.


== 3D Structures of Arabinanase ==
== 3D Structures of Arabinanase ==
[[Arabinanase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*Endo-arabinanase
 
**[[2x8f]], [[1uv4]] – BsEndo-Ara – ''Bacillus subtilis''<br />
**[[2x8t]], [[4cot]] - BsEndo-Ara (mutant) <br />
**[[3cu9]], [[1wl7]], [[5ho0]], [[5ho2]], [[5hp6]] – GsEndo-Ara – ''Geobacillus stearothermophilus''<br />
**[[3d5y]], [[3d60]] - GsEndo-Ara (mutant) <br />
**[[1gyd]] – CcEndo-Ara – ''Cellvibrio cellulose''<br />
**[[1gyh]] - CcEndo-Ara (mutant)<br />
**[[4kc7]], [[4kc8]] - TpEndo-Ara – ''Thermotoga petrophila''<br />
**[[4kca]], [[4kcb]] - Endo-Ara – bovine<br />
 
*Endo-arabinanase complex with substrate
 
**[[1gye]] - CcEndo-Ara (mutant) + arabinohexose<br />
**[[3d5z]], [[3d61]] - GsEndo-Ara (mutant) + arabinotriose<br />
**[[5ho9]] - GsEndo-Ara + arabinooctaose<br />
**[[5hof]] - GsEndo-Ara + pentaose<br />
**[[5hon]] - GsEndo-Ara + arabinotriose<br />
**[[2x8s]] - BsEndo-Ara (mutant) + arabinotriose<br />
 
*Exo-arabinanase
 
**[[3a71]] – PcExo-Ara residues 24-378 – ''Penicillium chrysogenum''<br />
**[[2w5n]] – GzExo-Ara – ''Gibberella zeae''
 
*Exo-arabinanase complex with substrate
 
**[[3a72]] - PcExo-Ara residues 24-378 + arabinobiose<br />
**[[2w5o]] - GzExo-Ara + arabinobiose<br />
 
}}


== References ==
== References ==

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky