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| [[Image:2x8f.png|left|200px|thumb|Crystal Structure of Arabinanase, [[2x8f]]]]
| | <StructureSection load='1gye' size='400' side='right' caption='Endo-arabinanase complex with arabinohexose (yellow) and Cl- ion (green) (PDB code [[1gye]])' scene='45/451702/Cv/1'> |
| {{STRUCTURE_3cu9| PDB=3cu9 | SIZE=400| SCENE= |right|CAPTION=1,5-α-arabinanase complex with Ca+2 ion (green) and glycerol, [[3cu9]] }}
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| '''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. See more details in [[Exo-alpha-1,5-L-Arabinanase]]. | |
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| __NOTOC__
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| | == Function == |
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| | '''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]]. '''Endo-arabinanase''' hydrolyzes from the N-terminal of the chain while '''exo-arabinanase''' hydrolyzes from the end of the chain. |
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| | == Structural highlights == |
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| | The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>. |
| | *<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>. |
| | *<scene name='45/451702/Cv/6'>Cl coordination site</scene>. |
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| == 3D Structures of Arabinanase == | | == 3D Structures of Arabinanase == |
| | [[Arabinanase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
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| ===Endo-arabinanase===
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| [[2x8f]]– BsEndo-Ara – ''Bacillus subtilis''<br />
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| [[2x8t]] - BsEndo-Ara (mutant) <br />
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| [[3cu9]], [[1wl7]] – GsEndo-Ara – ''Geobacillus stearothermophilus''<br />
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| [[3d5y]], [[3d60]] - GsEndo-Ara (mutant) <br />
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| [[1gyd]] – CcEndo-Ara – ''Cellvibrio cellulose''<br />
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| [[1gyh]] - CcEndo-Ara (mutant)<br />
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| [[4kc7]], [[4kc8]] - TpEndo-Ara – ''Thermotoga petrophila''<br />
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| [[4kca]] - Endo-Ara – bovine<br />
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| ===Endo-arabinanase complex with substrate===
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| [[1gye]] - CcEndo-Ara (mutant) + arabinohexose<br />
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| [[3d5z]], [[3d61]] - GsEndo-Ara (mutant) + arabinotriose<br />
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| [[2x8s]] - BsEndo-Ara (mutant) + arabinotriose<br />
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| ===Exo-arabinanase===
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| [[3a71]] – PcExo-Ara residues 24-378 – ''Penicillium chrysogenum''<br />
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| [[2w5n]] – GzExo-Ara – ''Gibberella zeae''
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| ===Exo-arabinanase complex with substrate===
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| [[3a72]] - PcExo-Ara residues 24-378 + arabinobiose<br />
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| [[2w5o]] - GzExo-Ara + arabinobiose<br />
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| | == References == |
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| | | <references/> |
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