Arabinanase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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-[[Image:2x8f.png|left|200px|thumb|Crystal Structure of Arabinanase, [[2x8f]]]]
+<StructureSection load='1gye' size='400' side='right' caption='Endo-arabinanase  complex with arabinohexose (yellow) and Cl- ion (green) (PDB code [[1gye]])' scene='45/451702/Cv/1'>
-{{STRUCTURE_3cu9| PDB=3cu9  | SIZE=400| SCENE= |right|CAPTION=Endo-arabinanase dimer from ''Bacillus subtilis'' with phosphates, calcium, MPD, glycerol, [[3cu9]] }}
-'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans. See more details in [[Exo-alpha-1,5-L-Arabinanase]].
-{{TOC limit|limit=2}}
+== Function ==
+'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].  '''Endo-arabinanase''' hydrolyzes from the N-terminal of the chain while '''exo-arabinanase''' hydrolyzes from the end of the chain.
+== Structural highlights ==
+The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>.
+*<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>.
+*<scene name='45/451702/Cv/6'>Cl coordination site</scene>.
 == 3D Structures of Arabinanase ==
+[[Arabinanase 3D structures]]
-===Endo-arabinanase===
+</StructureSection>
-[[2x8f]]– BsEndo-Ara – ''Bacillus subtilis''<br />
-[[2x8t]] - BsEndo-Ara (mutant) <br />
-[[3cu9]], [[1wl7]] – GsEndo-Ara – ''Geobacillus stearothermophilus''<br />
-[[3d5y]], [[3d60]] - GsEndo-Ara (mutant) <br />
-[[1gyd]] – CcEndo-Ara – ''Cellvibrio cellulose''<br />
-[[1gyh]] - CcEndo-Ara (mutant)
-===Endo-arabinanase complex with substrate===
-[[1gye]] - CcEndo-Ara (mutant) + arabinohexose<br />
-[[3d5z]], [[3d61]] - GsEndo-Ara (mutant) + arabinotriose<br />
-[[2x8s]] - BsEndo-Ara (mutant) + arabinotriose<br />
-===Exo-arabinanase===
-[[3a71]] – PcExo-Ara residues 24-378 – ''Penicillium chrysogenum''<br />
-[[2w5n]] – GzExo-Ara – ''Gibberella zeae''
-===Exo-arabinanase complex with substrate===
-[[3a72]] - PcExo-Ara residues 24-378 + arabinobiose<br />
-[[2w5o]] - GzExo-Ara + arabinobiose<br />
+== References ==
+  <references/>