Arabinanase: Difference between revisions

Latest revision as of 12:03, 27 May 2024

Function

Arabinanase (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans.^[1] See more details in Exo-alpha-1,5-L-Arabinanase. Endo-arabinanase hydrolyzes from the N-terminal of the chain while exo-arabinanase hydrolyzes from the end of the chain.

Structural highlights

The .^[2]. .

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3D Structures of Arabinanase

Arabinanase 3D structures

Endo-arabinanase complex with arabinohexose (yellow) and Cl- ion (green) (PDB code 1gye)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G, Shoham Y, Shoham G. Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrate. Biochem J. 2009 Jul 29;422(1):73-82. PMID:19505290 doi:10.1042/BJ20090180
↑ Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ. Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486 doi:10.1038/nsb835

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Michal Harel, Alexander Berchansky

[1] Alhassid A, Ben-David A, Tabachnikov O, Libster D, Naveh E, Zolotnitsky G, Shoham Y, Shoham G. Crystal structure of an inverting GH 43 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus complexed with its substrate. Biochem J. 2009 Jul 29;422(1):73-82. PMID:19505290 doi:10.1042/BJ20090180

[2] Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ. Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold. Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486 doi:10.1038/nsb835

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@@ Line 1: / Line 1: @@
-[[Image:2x8f.png|left|200px|thumb|Crystal Structure of Arabinanase, [[2x8f]]]]
+<StructureSection load='1gye' size='400' side='right' caption='Endo-arabinanase  complex with arabinohexose (yellow) and Cl- ion (green) (PDB code [[1gye]])' scene='45/451702/Cv/1'>
-{{STRUCTURE_2x8f|  PDB=2x8f  | SIZE=300| SCENE=Arabinanase/Arabinanase/1 |right|CAPTION=Endo-arabinanase from Bacillus subtilis with phosphates, calcium, MPD, glycerol, [[2x8f]] }}
-'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.
+== Function ==
-{{TOC limit|limit=2}}
+'''Arabinanase''' (Ara) hydrolyzes the α-1,5-linked L-arabinofuranoside backbone  of plant cell wall arabinans.<ref>PMID:19505290</ref> See more details in [[Exo-alpha-1,5-L-Arabinanase]].  '''Endo-arabinanase''' hydrolyzes from the N-terminal of the chain while '''exo-arabinanase''' hydrolyzes from the end of the chain.
+== Structural highlights ==
+The <scene name='45/451702/Cv/4'>active site of Ara is composed of 3 acidic residues</scene>.<ref>PMID:12198486</ref>. <scene name='45/451702/Cv/5'>Whole active site</scene>.
+*<scene name='45/451702/Cv/7'>Arabinohexose binding pocket</scene>.
+*<scene name='45/451702/Cv/6'>Cl coordination site</scene>.
+== 3D Structures of Arabinanase ==
+[[Arabinanase 3D structures]]
+</StructureSection>
-== 3D Structures of Arabinanase ==
+== References ==
+ <references/>