Anthrax protective antigen: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

@@ Line 1: / Line 1: @@
-{{STRUCTURE_1t6b|  PDB=1t6b  | SIZE=400| SCENE= |right|CAPTION=Anthrax protective antigen complex with anthrax toxin receptor domain A/I, tetraethylene glycol, Ca+2, Mn+2 and Na+ ions [[1t6b]] }}
+<StructureSection load='1acc' size='350' side='right' caption='Anthrax protective antigen (PA83) complex with Ca+2 ions (PDB code [[1acc]])' scene='48/485625/Cv/8'>
+__TOC__
+== Function ==
-<!--
+'''Anthrax protective antigen''' (PA) is the cell-binding part of the ''Bacillus anthracis'' anthrax toxin.  The anthrax toxin is composed of the PA, lethal factor and edema factor. The PA translocates the [[Anthrax Lethal Factor|lethal]] (LF) and [[Anthrax edema factor|edema]] factors (EF) into the cell.  The 83kD form of PA '''(PA83)''' binds to the anthrax toxin receptor.  A 20kD fragment (PA20) is cleaved off the PA83 and the remaining '''PA63''' assembles into ring-shaped oligomer which becomes a membrane pre-channel.  The PA pre-channel binds the lethal and edema factors allowing them to penetrate the membrane via pH gradient.
-Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
-Or use the four-green-boxes-button to insert scrollable text adjacent
-to a Jmol applet. Check out the other buttons as well!
--->
-'''Anthrax protective antigen''' (PA) is the cell-binding part of the ''Bacillus anthracis'' anthrax toxin.  The anthrax toxin is composed of the PA, lethal factor and edema factor.  The PA translocates the lethal and edema factors into the cell.  The 83kD form of PA (PA83) binds to the anthrax toxin receptor.  A 20kD fragment is cleaved off the PA83 and the remaining PA63 assembles into ring-shaped oligomer which becomes a membrane pre-channel.  PA domain 4 is important for receptor recognition and pore formation.  The PA pre-channel binds the lethal and edema factors allowing them to penetrate the membrane via pH gradient.
-'''PA83'''
+For more details see
-[[1acc]], [[3tew]], [[3q8a]], [[3q8b]] – PA<br />
+*[[Molecular Playground/Protective Antigen]]<br />
-[[3tex]], [[3tey]], [[3tez]], [[3q8c]], [[3q8e]] – PA (mutant)<br />
+*[[Anthrax Toxin Protein]]<br />
-[[3mhz]], [[3q8f]] – PA with fluoro-histidine<br />
+*[[Toxins]].
-[[1t6b]] – PA + anthrax toxin receptor A/I domain
-'''PA63'''
+== Structural highlights ==
-[[3hvd]] – PA63 (mutant)<br />
+PA contains 4 domains:<br />
-[[1tzo]] – PA63<br />
+* <scene name='48/485625/Cv/3'>Domain 1</scene> is the N terminal (in yellow).  It contains the furin protease cleavage site enabling the release of PA20.<br />
-[[1tzn]] – PA63 + anthrax toxin receptor VWA domain<br />
+* <scene name='48/485625/Cv/4'>Domain 2</scene> (in cyan) is the heptamerization domain of PA63.<br />
-[[3kwv]] – PA63 (mutant) + lethal factor
+* <scene name='48/485625/Cv/5'>Domain 3</scene> (in salmon) forms the heptameric pore with domain 2 and enabling LF and EF to penetrate the cell.<br />
+* <scene name='48/485625/Cv/6'>Domain 4</scene> (in magenta) is important for receptor recognition and pore formation. <br />
+<scene name='48/485625/Cv/11'>Click here to see Ca binding site</scene>. <ref>PMID:9039918</ref>
-'''PA domain 4'''
+==3D structures of anthrax protective antigen==
+[[Anthrax protective antigen 3D structures]]
-[[3ino]] – PA domain 4<br />
+</StructureSection>
-[[3etb]] – PA domain 4 + antibody (mutant)
+== References ==
+<references/>
+[[Category:Topic Page]]