2ayy: Difference between revisions
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==Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region== | ==Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region== | ||
<StructureSection load='2ayy' size='340' side='right'caption='[[2ayy | <StructureSection load='2ayy' size='340' side='right'caption='[[2ayy]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ayy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2ayy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AYY FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ayy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayy OCA], [https://pdbe.org/2ayy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ayy RCSB], [https://www.ebi.ac.uk/pdbsum/2ayy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ayy ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ayy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayy OCA], [https://pdbe.org/2ayy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ayy RCSB], [https://www.ebi.ac.uk/pdbsum/2ayy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ayy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/RCSC_ECOLI RCSC_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsC functions as a membrane-associated protein kinase that phosphorylates RcsD in response to environmental signals. The phosphoryl group is then transferred to the response regulator RcsB. RcsC has also phosphatase activity. The system controls expression of genes involved in colanic acid capsule synthesis, biofilm formation and cell division.<ref>PMID:10564486</ref> <ref>PMID:11758943</ref> <ref>PMID:11309126</ref> <ref>PMID:11807084</ref> <ref>PMID:13129944</ref> <ref>PMID:14651646</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bernhard | [[Category: Bernhard F]] | ||
[[Category: Dotsch | [[Category: Dotsch V]] | ||
[[Category: Koglin | [[Category: Koglin A]] | ||
[[Category: Lohr | [[Category: Lohr F]] | ||
[[Category: Rogov | [[Category: Rogov VV]] | ||
[[Category: Rogova | [[Category: Rogova NY]] | ||
Latest revision as of 14:19, 22 May 2024
Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker regionSolution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region
Structural highlights
FunctionRCSC_ECOLI Component of the Rcs signaling system, which controls transcription of numerous genes. RcsC functions as a membrane-associated protein kinase that phosphorylates RcsD in response to environmental signals. The phosphoryl group is then transferred to the response regulator RcsB. RcsC has also phosphatase activity. The system controls expression of genes involved in colanic acid capsule synthesis, biofilm formation and cell division.[1] [2] [3] [4] [5] [6] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe Rcs signalling pathway controls a variety of physiological functions like capsule synthesis, cell division or motility in prokaryotes. The Rcs regulation cascade, involving a multi-step phosphorelay between the two membrane-bound hybrid sensor kinases RcsC and RcsD and the global regulator RcsB, is, up to now, one of the most complicated regulatory systems in bacteria. To understand the structural basis of Rcs signal transduction, NMR spectroscopy was employed to determine the solution structure of the RcsC C terminus, possessing a phosphoreceiver domain (RcsC-PR), and a region previously described as a long linker between the histidine kinase domain of RcsC (RcsC-HK) and the RcsC-PR. We have found that the linker region comprises an independent structural domain of a new alpha/beta organization, which we named RcsC-ABL domain (Alpha/Beta/Loop). The ABL domain appears to be a conserved and unique structural element of RcsC-like kinases with no significant sequence homology to other proteins. The second domain of the C terminus, the RcsC-PR domain, represents a well-folded CheY-like phosphoreceiver domain with the central parallel beta-sheet covered with two alpha-helical layers on both sides. We have mapped the interaction of RcsC-ABL and RcsC-PR with the histidine phosphotransfer domain (HPt) of RcsD. In addition we have characterized the interaction with and the conformational effects of Mg2+ and the phosphorylation mimetic BeF(-)(3) on RcsC-ABL and RcsC-PR. A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.,Rogov VV, Rogova NY, Bernhard F, Koglin A, Lohr F, Dotsch V J Mol Biol. 2006 Nov 17;364(1):68-79. Epub 2006 Jul 29. PMID:17005198[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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