6to8: Difference between revisions
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==Neck of empty GTA particle computed with C12 symmetry== | |||
<StructureSection load='6to8' size='340' side='right'caption='[[6to8]], [[Resolution|resolution]] 3.36Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6to8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus_DE442 Rhodobacter capsulatus DE442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TO8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.36Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6to8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6to8 OCA], [https://pdbe.org/6to8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6to8 RCSB], [https://www.ebi.ac.uk/pdbsum/6to8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6to8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/D5ATZ4_RHOCB D5ATZ4_RHOCB] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. | |||
Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663<ref>PMID:32541663</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6to8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Portal protein 3D structures|Portal protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rhodobacter capsulatus DE442]] | |||
[[Category: Bardy P]] | |||
[[Category: Beatty JT]] | |||
[[Category: Fuzik T]] | |||
[[Category: Hrebik D]] | |||
[[Category: Pantucek R]] | |||
[[Category: Plevka P]] | |||
Latest revision as of 13:18, 22 May 2024
Neck of empty GTA particle computed with C12 symmetryNeck of empty GTA particle computed with C12 symmetry
Structural highlights
FunctionPublication Abstract from PubMedAlphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm. Structure and mechanism of DNA delivery of a gene transfer agent.,Bardy P, Fuzik T, Hrebik D, Pantucek R, Thomas Beatty J, Plevka P Nat Commun. 2020 Jun 15;11(1):3034. doi: 10.1038/s41467-020-16669-9. PMID:32541663[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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