6rn4: Difference between revisions
Jump to navigation
Jump to search
New page: '''Unreleased structure''' The entry 6rn4 is ON HOLD Authors: Deville, C., Saibil, H.R. Description: ClpB (DWB mutant) bound to casein in presence of ATPgammaS -state WT-2B [[Category:... |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==ClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-2B== | |||
<SX load='6rn4' size='340' side='right' viewer='molstar' caption='[[6rn4]], [[Resolution|resolution]] 4.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6rn4]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Escherichia_coli_HVH_50_(4-2593475) Escherichia coli HVH 50 (4-2593475)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RN4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rn4 OCA], [https://pdbe.org/6rn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rn4 RCSB], [https://www.ebi.ac.uk/pdbsum/6rn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rn4 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
AAA+ proteins form asymmetric hexameric rings that hydrolyze ATP and thread substrate proteins through a central channel via mobile substrate-binding pore loops. Understanding how ATPase and threading activities are regulated and intertwined is key to understanding the AAA+ protein mechanism. We studied the disaggregase ClpB, which contains tandem ATPase domains (AAA1, AAA2) and shifts between low and high ATPase and threading activities. Coiled-coil M-domains repress ClpB activity by encircling the AAA1 ring. Here, we determine the mechanism of ClpB activation by comparing ATPase mechanisms and cryo-EM structures of ClpB wild-type and a constitutively active ClpB M-domain mutant. We show that ClpB activation reduces ATPase cooperativity and induces a sequential mode of ATP hydrolysis in the AAA2 ring, the main ATPase motor. AAA1 and AAA2 rings do not work synchronously but in alternating cycles. This ensures high grip, enabling substrate threading via a processive, rope-climbing mechanism. | |||
Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor.,Deville C, Franke K, Mogk A, Bukau B, Saibil HR Cell Rep. 2019 Jun 18;27(12):3433-3446.e4. doi: 10.1016/j.celrep.2019.05.075. PMID:31216466<ref>PMID:31216466</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6rn4" style="background-color:#fffaf0;"></div> | ||
[[Category: Deville | |||
==See Also== | |||
*[[Heat Shock Protein structures|Heat Shock Protein structures]] | |||
*[[3D structures of ClpB|3D structures of ClpB]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Bos taurus]] | |||
[[Category: Large Structures]] | |||
[[Category: Deville C]] | |||
[[Category: Saibil HR]] | |||
Latest revision as of 13:12, 22 May 2024
ClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-2BClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-2B
| |||||||||