6bw1: Difference between revisions
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==Hendra virus W protein C-terminus in complex with Importin alpha 1== | ==Hendra virus W protein C-terminus in complex with Importin alpha 1== | ||
<StructureSection load='6bw1' size='340' side='right' caption='[[6bw1]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='6bw1' size='340' side='right'caption='[[6bw1]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6bw1]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6bw1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hendra_virus_horse/Australia/Hendra/1994 Hendra virus horse/Australia/Hendra/1994] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BW1 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bw1 OCA], [https://pdbe.org/6bw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bw1 RCSB], [https://www.ebi.ac.uk/pdbsum/6bw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bw1 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/W_HENDH W_HENDH] | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 21: | Line 20: | ||
==See Also== | ==See Also== | ||
*[[Importin|Importin]] | *[[Importin 3D structures|Importin 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Hendra virus horse/Australia/Hendra/1994]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Aragao | [[Category: Mus musculus]] | ||
[[Category: Basler | [[Category: Aragao D]] | ||
[[Category: Cross | [[Category: Basler CF]] | ||
[[Category: Edwards | [[Category: Cross EM]] | ||
[[Category: Forwood | [[Category: Edwards MR]] | ||
[[Category: Smith | [[Category: Forwood JK]] | ||
[[Category: Tsimbalyuk | [[Category: Smith KM]] | ||
[[Category: Tsimbalyuk S]] | |||
Latest revision as of 13:07, 22 May 2024
Hendra virus W protein C-terminus in complex with Importin alpha 1Hendra virus W protein C-terminus in complex with Importin alpha 1
Structural highlights
FunctionPublication Abstract from PubMedSeven human isoforms of importin alpha mediate nuclear import of cargo in a tissue- and isoform-specific manner. How nuclear import adaptors differentially interact with cargo harbouring the same nuclear localisation signal (NLS) remains poorly understood, as the NLS recognition region is highly conserved. Here, we provide a structural basis for the nuclear import specificity of W proteins in Hendra and Nipah viruses. We determine the structural interfaces of these cargo bound to importin alpha1 and alpha3, identifying a 2.4-fold more extensive interface and > 50-fold higher binding affinity for importin alpha3. Through the design of importin alpha1 and alpha3 chimeric and mutant proteins, together with structures of cargo-free importin alpha1 and alpha3 isoforms, we establish that the molecular basis of specificity resides in the differential positioning of the armadillo repeats 7 and 8. Overall, our study provides mechanistic insights into a range of important nucleocytoplasmic transport processes reliant on isoform adaptor specificity. Structural basis for importin alpha 3 specificity of W proteins in Hendra and Nipah viruses.,Smith KM, Tsimbalyuk S, Edwards MR, Cross EM, Batra J, Soares da Costa TP, Aragao D, Basler CF, Forwood JK Nat Commun. 2018 Sep 12;9(1):3703. doi: 10.1038/s41467-018-05928-5. PMID:30209309[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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