5bkh: Difference between revisions

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-'''Unreleased structure'''
-The entry 5bkh is ON HOLD  until Paper Publication
+==The splicing activity and an alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein==
+<StructureSection load='5bkh' size='340' side='right'caption='[[5bkh]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5bkh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BKH FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bkh OCA], [https://pdbe.org/5bkh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bkh RCSB], [https://www.ebi.ac.uk/pdbsum/5bkh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bkh ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DP2L_PYRHO DP2L_PYRHO] Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Protein splicing is a post-translational process by which an intein catalyzes its own excision from flanking polypeptides, or exteins, concomitant with extein ligation. Many inteins have nested homing endonuclease domains that facilitate their propagation into intein-less alleles, whereas other inteins lack the homing endonuclease (HEN) and are called mini-inteins. The mini-intein that interrupts the DNA PolII of Pyrococcus horikoshii has a linker region in place of the HEN domain that is shorter than the linker in a closely related intein from Pyrococcus abyssi. The P. horikoshii PolII intein requires a higher temperature for catalytic activity and is more stable to digestion by the thermostable protease thermolysin, suggesting that it is more rigid than the P. abyssi intein. We solved a crystal structure of the intein precursor that revealed a domain-swapped dimer. Inteins found as domain swapped dimers have been shown to promote intein-mediated protein alternative splicing, but the solved P. horikoshii PolII intein structure has an active site unlikely to be catalytically competent.
-Authors: Li, Z., Li, H.
+An alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein.,Williams JE, Jaramillo MV, Li Z, Zhao J, Wang C, Li H, Mills KV Sci Rep. 2021 Jun 3;11(1):11680. doi: 10.1038/s41598-021-91090-w. PMID:34083592<ref>PMID:34083592</ref>
-Description: The splicing activity and an alternative domain-swapped structure of the Pyrococcus horikoshii PolII mini-intein
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Li, H]]
+<div class="pdbe-citations 5bkh" style="background-color:#fffaf0;"></div>
-[[Category: Li, Z]]
+==See Also==
+*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Pyrococcus horikoshii OT3]]
+[[Category: Li H]]
+[[Category: Li Z]]