4j31: Difference between revisions

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-{{STRUCTURE_4j31|  PDB=4j31  |  SCENE=  }}
-===Crystal Structure of kynurenine 3-monooxygenase (KMO-396Prot)===
-==Function==
+==Crystal Structure of kynurenine 3-monooxygenase (KMO-396Prot)==
-[[http://www.uniprot.org/uniprot/KMO_YEAST KMO_YEAST]] Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.<ref>PMID:12062417</ref> <ref>PMID:15806102</ref>
+<StructureSection load='4j31' size='340' side='right'caption='[[4j31]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4j31]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J31 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j31 OCA], [https://pdbe.org/4j31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j31 RCSB], [https://www.ebi.ac.uk/pdbsum/4j31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j31 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KMO_YEAST KMO_YEAST] Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid.<ref>PMID:12062417</ref> <ref>PMID:15806102</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Inhibition of kynurenine 3-monooxygenase (KMO), an enzyme in the eukaryotic tryptophan catabolic pathway (that is, kynurenine pathway), leads to amelioration of Huntington's-disease-relevant phenotypes in yeast, fruitfly and mouse models, as well as in a mouse model of Alzheimer's disease. KMO is a flavin adenine dinucleotide (FAD)-dependent monooxygenase and is located in the outer mitochondrial membrane where it converts l-kynurenine to 3-hydroxykynurenine. Perturbations in the levels of kynurenine pathway metabolites have been linked to the pathogenesis of a spectrum of brain disorders, as well as cancer and several peripheral inflammatory conditions. Despite the importance of KMO as a target for neurodegenerative disease, the molecular basis of KMO inhibition by available lead compounds has remained unknown. Here we report the first crystal structure of Saccharomyces cerevisiae KMO, in the free form and in complex with the tight-binding inhibitor UPF 648. UPF 648 binds close to the FAD cofactor and perturbs the local active-site structure, preventing productive binding of the substrate l-kynurenine. Functional assays and targeted mutagenesis reveal that the active-site architecture and UPF 648 binding are essentially identical in human KMO, validating the yeast KMO-UPF 648 structure as a template for structure-based drug design. This will inform the search for new KMO inhibitors that are able to cross the blood-brain barrier in targeted therapies against neurodegenerative diseases such as Huntington's, Alzheimer's and Parkinson's diseases.
-==About this Structure==
+Structural basis of kynurenine 3-monooxygenase inhibition.,Amaral M, Levy C, Heyes DJ, Lafite P, Outeiro TF, Giorgini F, Leys D, Scrutton NS Nature. 2013 Apr 18;496(7445):382-5. doi: 10.1038/nature12039. Epub 2013 Apr 10. PMID:23575632<ref>PMID:23575632</ref>
-[[4j31]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J31 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<references group="xtra"/><references/>
+</div>
-[[Category: Kynurenine 3-monooxygenase]]
+<div class="pdbe-citations 4j31" style="background-color:#fffaf0;"></div>
-[[Category: Saccharomyces cerevisiae]]
-[[Category: Amaral, M.]]
+==See Also==
-[[Category: Giorgini, F.]]
+*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
-[[Category: Heyes, D J.]]
+== References ==
-[[Category: Lafite, P.]]
+<references/>
-[[Category: Levy, C.]]
+__TOC__
-[[Category: Leys, D.]]
+</StructureSection>
-[[Category: Outeiro, T F.]]
+[[Category: Large Structures]]
-[[Category: Scrutton, N S.]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Monooxygenase kynuramine]]
+[[Category: Amaral M]]
-[[Category: Oxidoreductase]]
+[[Category: Giorgini F]]
+[[Category: Heyes DJ]]
+[[Category: Lafite P]]
+[[Category: Levy C]]
+[[Category: Leys D]]
+[[Category: Outeiro TF]]
+[[Category: Scrutton NS]]