1ry4: Difference between revisions

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OCA

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-[[Image:1ry4.jpg|left|200px]]<br /><applet load="1ry4" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ry4" />
-'''NMR Structure of the CRIB-PDZ module of Par-6'''<br />
-==Overview==
+==NMR Structure of the CRIB-PDZ module of Par-6==
-Regulation of protein interaction domains is required for cellular, signaling dynamics. Here, we show that the PDZ protein interaction domain, from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42., Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the, affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately, 13-fold. Par-6 PDZ regulation is required for function as mutational, disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in, polarized MDCK epithelial cells. Structural analysis reveals that the free, PDZ domain has several deviations from the canonical PDZ conformation that, account for its low ligand affinity. Regulation results from a, Cdc42-induced conformational transition in the CRIB-PDZ module that causes, the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled, CRIB and PDZ architecture of Par-6 reveals how simple binding domains can, be combined to yield complex regulation.
+<StructureSection load='1ry4' size='340' side='right'caption='[[1ry4]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ry4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RY4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ry4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ry4 OCA], [https://pdbe.org/1ry4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ry4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ry4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ry4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/O97111_DROME O97111_DROME]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1ry4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ry4 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Regulation of protein interaction domains is required for cellular signaling dynamics. Here, we show that the PDZ protein interaction domain from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42. Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately 13-fold. Par-6 PDZ regulation is required for function as mutational disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in polarized MDCK epithelial cells. Structural analysis reveals that the free PDZ domain has several deviations from the canonical PDZ conformation that account for its low ligand affinity. Regulation results from a Cdc42-induced conformational transition in the CRIB-PDZ module that causes the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled CRIB and PDZ architecture of Par-6 reveals how simple binding domains can be combined to yield complex regulation.
-==About this Structure==
+Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition.,Peterson FC, Penkert RR, Volkman BF, Prehoda KE Mol Cell. 2004 Mar 12;13(5):665-76. PMID:15023337<ref>PMID:15023337</ref>
-RY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition., Peterson FC, Penkert RR, Volkman BF, Prehoda KE, Mol Cell. 2004 Mar 12;13(5):665-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15023337 15023337]
+</div>
+<div class="pdbe-citations 1ry4" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Penkert, R.R.]]
+[[Category: Penkert RR]]
-[[Category: Peterson, F.C.]]
+[[Category: Peterson FC]]
-[[Category: Prehoda, K.E.]]
+[[Category: Prehoda KE]]
-[[Category: Volkman, B.F.]]
+[[Category: Volkman BF]]
-[[Category: cdc-42]]
-[[Category: cell polarization]]
-[[Category: crib]]
-[[Category: pdz]]
-[[Category: polarity adaptor complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:29:53 2007''