1n3k: Difference between revisions

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-[[Image:1n3k.png|left|200px]]
-{{STRUCTURE_1n3k|  PDB=1n3k  |  SCENE=  }}
+==Solution structure of phosphoprotein enriched in astrocytes 15 kDa (PEA-15)==
+<StructureSection load='1n3k' size='340' side='right'caption='[[1n3k]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1n3k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3K FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3k OCA], [https://pdbe.org/1n3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3k RCSB], [https://www.ebi.ac.uk/pdbsum/1n3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3k ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PEA15_CRIGR PEA15_CRIGR] Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface (By similarity). Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm.<ref>PMID:9852038</ref> <ref>PMID:10982386</ref> <ref>PMID:14506247</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3k ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+PEA-15 is a multifunctional protein that modulates signaling pathways which control cell proliferation and cell death. In particular, PEA-15 regulates the actions of the ERK MAP kinase cascade by binding to ERK and altering its subcellular localization. The three-dimensional structure of PEA-15 has been determined using NMR spectroscopy and its interaction with ERK defined by characterization of mutants that modulate ERK function. PEA-15 is composed of an N-terminal death effector domain (DED) and a C-terminal tail of irregular structure. NMR 'footprinting' and mutagenesis identified elements of both the DED and tail that are required for ERK binding. Comparison of the DED-binding surface for ERK2 with the death domain (DD)-binding surface of Drosophila Tube revealed an unexpected similarity between the interaction modes of the DD and DED motifs in these proteins. Despite a lack of functional or sequence similarity between PEA-15 and Tube, these proteins utilize a common surface of the structurally similar DD and DED to recognize functionally diverse targets.
-===Solution structure of phosphoprotein enriched in astrocytes 15 kDa (PEA-15)===
+Recognition of ERK MAP kinase by PEA-15 reveals a common docking site within the death domain and death effector domain.,Hill JM, Vaidyanathan H, Ramos JW, Ginsberg MH, Werner MH EMBO J. 2002 Dec 2;21(23):6494-504. PMID:12456656<ref>PMID:12456656</ref>
-{{ABSTRACT_PUBMED_12456656}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1n3k" style="background-color:#fffaf0;"></div>
-[[1n3k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3K OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:012456656</ref><references group="xtra"/>
+</StructureSection>
 [[Category: Cricetulus griseus]]
-[[Category: Ginsberg, M H.]]
+[[Category: Large Structures]]
-[[Category: Hill, J M.]]
+[[Category: Ginsberg MH]]
-[[Category: Ramos, J W.]]
+[[Category: Hill JM]]
-[[Category: Vaidyanathan, H.]]
+[[Category: Ramos JW]]
-[[Category: Werner, M H.]]
+[[Category: Vaidyanathan H]]
-[[Category: Apoptosis]]
+[[Category: Werner MH]]
-[[Category: Death effector domain]]
-[[Category: Six helix bundle]]