1isk: Difference between revisions

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-{{Seed}}
-[[Image:1isk.png|left|200px]]
-<!--
+==3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES==
-The line below this paragraph, containing "STRUCTURE_1isk", creates the "Structure Box" on the page.
+<StructureSection load='1isk' size='340' side='right'caption='[[1isk]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1isk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ISK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ISK FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1isk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1isk OCA], [https://pdbe.org/1isk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1isk RCSB], [https://www.ebi.ac.uk/pdbsum/1isk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1isk ProSAT]</span></td></tr>
-{{STRUCTURE_1isk|  PDB=1isk  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SDIS_COMTE SDIS_COMTE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/1isk_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1isk ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.
-===3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES===
+Solution structure of 3-oxo-delta5-steroid isomerase.,Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF Science. 1997 Apr 18;276(5311):415-8. PMID:9103200<ref>PMID:9103200</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1isk" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_9103200}}, adds the Publication Abstract to the page
+*[[Ketosteroid Isomerase|Ketosteroid Isomerase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 9103200 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9103200}}
+__TOC__
+</StructureSection>
-==About this Structure==
-ISK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ISK OCA].
-==Reference==
-<ref group="xtra">PMID:9103200</ref><references group="xtra"/>
 [[Category: Comamonas testosteroni]]
-[[Category: Steroid Delta-isomerase]]
+[[Category: Large Structures]]
-[[Category: Brothers, P.]]
+[[Category: Brothers P]]
-[[Category: Ebrahimian, S.]]
+[[Category: Ebrahimian S]]
-[[Category: Perez-Alvarado, G C.]]
+[[Category: Perez-Alvarado GC]]
-[[Category: Pollack, R M.]]
+[[Category: Pollack RM]]
-[[Category: Summers, M F.]]
+[[Category: Summers MF]]
-[[Category: Thornburg, L D.]]
+[[Category: Thornburg LD]]
-[[Category: Wu, Z R.]]
+[[Category: Wu ZR]]
-[[Category: Zawrotny, M E.]]
+[[Category: Zawrotny ME]]
-[[Category: 3-ketosteroid]]
-[[Category: Isomerase]]
-[[Category: Ksi]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:48:17 2009''