1bb8: Difference between revisions

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-[[Image:1bb8.gif|left|200px]]
-<!--
-The line below this paragraph, containing "STRUCTURE_1bb8", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
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-{{STRUCTURE_1bb8|  PDB=1bb8  |  SCENE=  }}
-'''N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, 25 STRUCTURES'''
+==N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, 25 STRUCTURES==
+<StructureSection load='1bb8' size='340' side='right'caption='[[1bb8]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1bb8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BB8 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bb8 OCA], [https://pdbe.org/1bb8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bb8 RCSB], [https://www.ebi.ac.uk/pdbsum/1bb8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bb8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TNR6_ENTFL TNR6_ENTFL]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/1bb8_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bb8 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The integrase family of site-specific recombinases catalyze a diverse array of DNA rearrangements in archaebacteria, eubacteria and yeast. The solution structure of the DNA binding domain of the integrase protein from the conjugative transposon Tn916 has been determined using NMR spectroscopy. The structure provides the first insights into distal site DNA binding by a site-specific integrase and reveals that the N-terminal domain is structurally similar to the double stranded RNA binding domain (dsRBD). The results of chemical shift mapping experiments suggest that the integrase protein interacts with DNA using residues located on the face of its three stranded beta-sheet. This surface differs from the proposed RNA binding surface in dsRBDs, suggesting that different surfaces on the same protein fold can be used to bind DNA and RNA.
-==Overview==
+Site-specific DNA binding using a variation of the double stranded RNA binding motif.,Connolly KM, Wojciak JM, Clubb RT Nat Struct Biol. 1998 Jul;5(7):546-50. PMID:9665166<ref>PMID:9665166</ref>
-The integrase family of site-specific recombinases catalyze a diverse array of DNA rearrangements in archaebacteria, eubacteria and yeast. The solution structure of the DNA binding domain of the integrase protein from the conjugative transposon Tn916 has been determined using NMR spectroscopy. The structure provides the first insights into distal site DNA binding by a site-specific integrase and reveals that the N-terminal domain is structurally similar to the double stranded RNA binding domain (dsRBD). The results of chemical shift mapping experiments suggest that the integrase protein interacts with DNA using residues located on the face of its three stranded beta-sheet. This surface differs from the proposed RNA binding surface in dsRBDs, suggesting that different surfaces on the same protein fold can be used to bind DNA and RNA.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-BB8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB8 OCA].
+</div>
+<div class="pdbe-citations 1bb8" style="background-color:#fffaf0;"></div>
-==Reference==
+==See Also==
-Site-specific DNA binding using a variation of the double stranded RNA binding motif., Connolly KM, Wojciak JM, Clubb RT, Nat Struct Biol. 1998 Jul;5(7):546-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9665166 9665166]
+*[[Integrase|Integrase]]
+*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Enterococcus faecalis]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Clubb, R T.]]
+[[Category: Clubb RT]]
-[[Category: Connolly, K M.]]
+[[Category: Connolly KM]]
-[[Category: Wojciak, J M.]]
+[[Category: Wojciak JM]]
-[[Category: Dna binding]]
-[[Category: Integrase]]
-[[Category: Transposition]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:17:59 2008''