1aw6: Difference between revisions

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-[[Image:1aw6.gif|left|200px]]<br />
-<applet load="1aw6" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1aw6" />
-'''GAL4 (CD), NMR, 24 STRUCTURES'''<br />
-==Overview==
+==GAL4 (CD), NMR, 24 STRUCTURES==
-We have refined the solution structure of cadmium-bound GAL4 and present, its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to, the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/-, 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H), coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the, bimetal-thiolate cluster in GAL4. We conclude that the structural, parameters that correlate to 3J(113Cd,1H) are complex.
+<StructureSection load='1aw6' size='340' side='right'caption='[[1aw6]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1aw6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=125d 125d]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AW6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw6 OCA], [https://pdbe.org/1aw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1aw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aw6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GAL4_YEAST GAL4_YEAST] This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aw/1aw6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aw6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex.
-==About this Structure==
+Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination.,Baleja JD, Thanabal V, Wagner G J Biomol NMR. 1997 Dec;10(4):397-401. PMID:9460244<ref>PMID:9460244</ref>
-AW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 125D. Structure known Active Sites: CD4 and CD5. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AW6 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination., Baleja JD, Thanabal V, Wagner G, J Biomol NMR. 1997 Dec;10(4):397-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9460244 9460244]
+</div>
+<div class="pdbe-citations 1aw6" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Baleja JD]]
-[[Category: Baleja, J.D.]]
+[[Category: Wagner G]]
-[[Category: Wagner, G.]]
-[[Category: CD]]
-[[Category: dna-binding]]
-[[Category: transcription regulation]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 12:42:56 2007''