1aj3: Difference between revisions

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-{{Seed}}
-[[Image:1aj3.png|left|200px]]
-<!--
+==SOLUTION STRUCTURE OF THE SPECTRIN REPEAT, NMR, 20 STRUCTURES==
-The line below this paragraph, containing "STRUCTURE_1aj3", creates the "Structure Box" on the page.
+<StructureSection load='1aj3' size='340' side='right'caption='[[1aj3]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1aj3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AJ3 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aj3 OCA], [https://pdbe.org/1aj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aj3 RCSB], [https://www.ebi.ac.uk/pdbsum/1aj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aj3 ProSAT]</span></td></tr>
-{{STRUCTURE_1aj3|  PDB=1aj3  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/1aj3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aj3 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cytoskeletal proteins belonging to the spectrin family have an elongated structure composed of repetitive units. The three-dimensional solution structure of the 16th repeat from chicken brain alpha-spectrin (R16) has been determined by NMR spectroscopy and distance geometry-simulated annealing calculations. We used a total of 1035 distance restraints, which included 719 NOE-based values obtained by applying the ambiguous restraints for iterative assignment (ARIA) method. In addition, we performed a direct refinement against 1H-chemical shifts. The final ensemble of 20 structures shows an average RMSD of 1.52 A from the mean for the backbone atoms, excluding loops and N and C termini. R16 is made up of three antiparallel alpha-helices separated by two loops, and folds into a left-handed coiled-coil. The basic unit of spectrin is an antiparallel heterodimer composed of two homologous chains, beta and alpha. These assemble a tetramer via a mechanism that relies on the completion of a single repeat by association of the partial repeats located at the C terminus of the beta-chain (two helices) and at the N terminus of the alpha-chain (one helix). This tetramer is the assemblage able to cross-link actin filaments. Model building by homology of the "tetramerization" repeat from human erythrocyte spectrin illuminates the possible role of point mutations which cause hemolytic anemias.
-===SOLUTION STRUCTURE OF THE SPECTRIN REPEAT, NMR, 20 STRUCTURES===
+Solution structure of the spectrin repeat: a left-handed antiparallel triple-helical coiled-coil.,Pascual J, Pfuhl M, Walther D, Saraste M, Nilges M J Mol Biol. 1997 Oct 31;273(3):740-51. PMID:9356261<ref>PMID:9356261</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1aj3" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_9356261}}, adds the Publication Abstract to the page
+*[[Spectrin 3D structures|Spectrin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 9356261 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9356261}}
+__TOC__
+</StructureSection>
-==About this Structure==
-AJ3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ3 OCA].
-==Reference==
-Solution structure of the spectrin repeat: a left-handed antiparallel triple-helical coiled-coil., Pascual J, Pfuhl M, Walther D, Saraste M, Nilges M, J Mol Biol. 1997 Oct 31;273(3):740-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9356261 9356261]
 [[Category: Gallus gallus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Nilges, M.]]
+[[Category: Nilges M]]
-[[Category: Pascual, J.]]
+[[Category: Pascual J]]
-[[Category: Pfuhl, M.]]
+[[Category: Pfuhl M]]
-[[Category: Saraste, M.]]
+[[Category: Saraste M]]
-[[Category: Walther, D.]]
+[[Category: Walther D]]
-[[Category: Actin-binding]]
-[[Category: Calcium-binding]]
-[[Category: Calmodulin-binding]]
-[[Category: Capping protein]]
-[[Category: Coiled-coil]]
-[[Category: Cytoskeleton]]
-[[Category: Duplication]]
-[[Category: Elasticity]]
-[[Category: Membrane skeleton]]
-[[Category: Repeat]]
-[[Category: Sh3 domain]]
-[[Category: Spectrin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:57:23 2008''