1ah9: Difference between revisions

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-{{Seed}}
-[[Image:1ah9.png|left|200px]]
-<!--
+==THE STRUCTURE OF THE TRANSLATIONAL INITIATION FACTOR IF1 FROM ESCHERICHIA COLI, NMR, 19 STRUCTURES==
-The line below this paragraph, containing "STRUCTURE_1ah9", creates the "Structure Box" on the page.
+<StructureSection load='1ah9' size='340' side='right'caption='[[1ah9]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1ah9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AH9 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ah9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ah9 OCA], [https://pdbe.org/1ah9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ah9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ah9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ah9 ProSAT]</span></td></tr>
-{{STRUCTURE_1ah9|  PDB=1ah9  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IF1_ECOLI IF1_ECOLI] No specific function has so far been attributed to this initiation factor; however, it seems to stimulate more or less all the activities of the other two initiation factors, IF-2 and IF-3.<ref>PMID:376343</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/1ah9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ah9 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The structure of the translational initiation factor IF1 from Escherichia coli has been determined with multidimensional NMR spectroscopy. Using 1041 distance and 78 dihedral constraints, 40 distance geometry structures were calculated, which were refined by restrained molecular dynamics. From this set, 19 structures were selected, having low constraint energy and few constraint violations. The ensemble of 19 structures displays a root-mean-square deviation versus the average of 0.49 A for the backbone atoms and 1.12 A for all atoms for residues 6-36 and 46-67. The structure of IF1 is characterized by a five-stranded beta-barrel. The loop connecting strands three and four contains a short 3(10) helix but this region shows considerably higher flexibility than the beta-barrel. The fold of IF1 is very similar to that found in the bacterial cold shock proteins CspA and CspB, the N-terminal domain of aspartyl-tRNA synthetase and the staphylococcal nuclease, and can be identified as the oligomer-binding motif. Several proteins of this family are nucleic acid-binding proteins. This suggests that IF1 plays its role in the initiation of protein synthesis by nucleic acid interactions. Specific changes of NMR signals of IF1 upon titration with 30S ribosomal subunit identifies several residues that are involved in the interaction with ribosomes.
-===THE STRUCTURE OF THE TRANSLATIONAL INITIATION FACTOR IF1 FROM ESCHERICHIA COLI, NMR, 19 STRUCTURES===
+The structure of the translational initiation factor IF1 from E.coli contains an oligomer-binding motif.,Sette M, van Tilborg P, Spurio R, Kaptein R, Paci M, Gualerzi CO, Boelens R EMBO J. 1997 Mar 17;16(6):1436-43. PMID:9135158<ref>PMID:9135158</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_9135158}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1ah9" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 9135158 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9135158}}
+__TOC__
+</StructureSection>
-==About this Structure==
-AH9 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AH9 OCA].
-==Reference==
-<ref group="xtra">PMID:9135158</ref><references group="xtra"/>
 [[Category: Escherichia coli]]
-[[Category: Boelens, R.]]
+[[Category: Large Structures]]
-[[Category: Gualerzi, C O.]]
+[[Category: Boelens R]]
-[[Category: Kaptein, R.]]
+[[Category: Gualerzi CO]]
-[[Category: Paci, M.]]
+[[Category: Kaptein R]]
-[[Category: Sette, M.]]
+[[Category: Paci M]]
-[[Category: Spurio, R.]]
+[[Category: Sette M]]
-[[Category: Tilborg, P Van.]]
+[[Category: Spurio R]]
-[[Category: Ob fold]]
+[[Category: Van Tilborg P]]
-[[Category: Protein-rna interaction]]
-[[Category: Ribosome binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:09:27 2009''