2ahr: Difference between revisions

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-[[Image:2ahr.png|left|200px]]
-<!--
+==Crystal Structures of 1-Pyrroline-5-Carboxylate Reductase from Human Pathogen Streptococcus pyogenes==
-The line below this paragraph, containing "STRUCTURE_2ahr", creates the "Structure Box" on the page.
+<StructureSection load='2ahr' size='340' side='right'caption='[[2ahr]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ahr]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_M1_GAS Streptococcus pyogenes M1 GAS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AHR FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-{{STRUCTURE_2ahr|  PDB=2ahr  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ahr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ahr OCA], [https://pdbe.org/2ahr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ahr RCSB], [https://www.ebi.ac.uk/pdbsum/2ahr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ahr ProSAT], [https://www.topsan.org/Proteins/MCSG/2ahr TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9A1S9_STRP1 Q9A1S9_STRP1]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/2ahr_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ahr ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+L-proline is an amino acid that plays an important role in proteins uniquely contributing to protein folding, structure, and stability, and this amino acid serves as a sequence-recognition motif. Proline biosynthesis can occur via two pathways, one from glutamate and the other from arginine. In both pathways, the last step of biosynthesis, the conversion of delta1-pyrroline-5-carboxylate (P5C) to L-proline, is catalyzed by delta1-pyrroline-5-carboxylate reductase (P5CR) using NAD(P)H as a cofactor. We have determined the first crystal structure of P5CR from two human pathogens, Neisseria meningitides and Streptococcus pyogenes, at 2.0 angstroms and 2.15 angstroms resolution, respectively. The catalytic unit of P5CR is a dimer composed of two domains, but the biological unit seems to be species-specific. The N-terminal domain of P5CR is an alpha/beta/alpha sandwich, a Rossmann fold. The C-terminal dimerization domain is rich in alpha-helices and shows domain swapping. Comparison of the native structure of P5CR to structures complexed with L-proline and NADP+ in two quite different primary sequence backgrounds provides unique information about key functional features: the active site and the catalytic mechanism. The inhibitory L-proline has been observed in the crystal structure.
-===Crystal Structures of 1-Pyrroline-5-Carboxylate Reductase from Human Pathogen Streptococcus pyogenes===
+Crystal structures of delta1-pyrroline-5-carboxylate reductase from human pathogens Neisseria meningitides and Streptococcus pyogenes.,Nocek B, Chang C, Li H, Lezondra L, Holzle D, Collart F, Joachimiak A J Mol Biol. 2005 Nov 18;354(1):91-106. Epub 2005 Sep 2. PMID:16233902<ref>PMID:16233902</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ahr" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_16233902}}, adds the Publication Abstract to the page
+*[[Pyrroline-5-carboxylate reductase|Pyrroline-5-carboxylate reductase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 16233902 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16233902}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[2ahr]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AHR OCA].
+[[Category: Streptococcus pyogenes M1 GAS]]
+[[Category: Holzle D]]
-==Reference==
+[[Category: Joachimiak A]]
-<ref group="xtra">PMID:016233902</ref><references group="xtra"/>
+[[Category: Lezondra L]]
-[[Category: Pyrroline-5-carboxylate reductase]]
+[[Category: Nocek B]]
-[[Category: Streptococcus pyogenes]]
-[[Category: Holzle, D.]]
-[[Category: Joachimiak, A.]]
-[[Category: Lezondra, L.]]
-[[Category: MCSG, Midwest Center for Structural Genomics.]]
-[[Category: Nocek, B.]]
-[[Category: 1-pyrroline-5-carboxylate reductase]]
-[[Category: Doain swapping]]
-[[Category: Human pathogen]]
-[[Category: Mcsg]]
-[[Category: Midwest center for structural genomic]]
-[[Category: Oxidoreductase]]
-[[Category: Proline biosynthesis]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Pyrroline reductase]]
-[[Category: Pyrroline-5-carboxylate reductase]]
-[[Category: Rossmann fold]]
-[[Category: Streptococcus pyogene]]
-[[Category: Structural genomic]]