2a56: Difference between revisions

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-{{Seed}}
-[[Image:2a56.png|left|200px]]
-<!--
+==fluorescent protein asFP595, A143S, on-state, 5min irradiation==
-The line below this paragraph, containing "STRUCTURE_2a56", creates the "Structure Box" on the page.
+<StructureSection load='2a56' size='340' side='right'caption='[[2a56]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2a56]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anemonia_sulcata Anemonia sulcata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A56 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A56 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-{{STRUCTURE_2a56|  PDB=2a56  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a56 OCA], [https://pdbe.org/2a56 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a56 RCSB], [https://www.ebi.ac.uk/pdbsum/2a56 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a56 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NFCP_ANESU NFCP_ANESU] Pigment protein that is intensely purple in color.<ref>PMID:10852900</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/2a56_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a56 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Proteins that can be reversibly photoswitched between a fluorescent and a nonfluorescent state bear enormous potential in diverse fields, such as data storage, in vivo protein tracking, and subdiffraction resolution light microscopy. However, these proteins could hitherto not live up to their full potential because the molecular switching mechanism is not resolved. Here, we clarify the molecular photoswitching mechanism of asFP595, a green fluorescent protein (GFP)-like protein that can be transferred from a nonfluorescent "off" to a fluorescent "on" state and back again, by green and blue light, respectively. To this end, we establish reversible photoswitching of fluorescence in whole protein crystals and show that the switching kinetics in the crystal is identical with that in solution. Subsequent x-ray analysis demonstrated that upon the absorption of a green photon, the chromophore isomerizes from a trans (off) to a cis (on) state. Molecular dynamics calculations suggest that isomerization occurs through a bottom hula twist mechanism with concomitant rotation of both bonds of the chromophoric methine ring bridge. This insight into the switching mechanism should facilitate the targeted design of photoswitchable proteins. Reversible photoswitching of the protein chromophore system within intact crystals also constitutes a step toward the use of fluorescent proteins in three-dimensional data recording.
-===fluorescent protein asFP595, A143S, on-state, 5min irradiation===
+Structure and mechanism of the reversible photoswitch of a fluorescent protein.,Andresen M, Wahl MC, Stiel AC, Grater F, Schafer LV, Trowitzsch S, Weber G, Eggeling C, Grubmuller H, Hell SW, Jakobs S Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13070-4. Epub 2005 Aug 31. PMID:16135569<ref>PMID:16135569</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_16135569}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2a56" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 16135569 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_16135569}}
+__TOC__
+</StructureSection>
-==About this Structure==
-A56 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Anemonia_sulcata Anemonia sulcata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A56 OCA].
-==Reference==
-<ref group="xtra">PMID:16135569</ref><references group="xtra"/>
 [[Category: Anemonia sulcata]]
-[[Category: Andresen, M.]]
+[[Category: Large Structures]]
-[[Category: Eggeling, C.]]
+[[Category: Andresen M]]
-[[Category: Graeter, F.]]
+[[Category: Eggeling C]]
-[[Category: Grubmueller, H.]]
+[[Category: Graeter F]]
-[[Category: Hell, S W.]]
+[[Category: Grubmueller H]]
-[[Category: Jakobs, S.]]
+[[Category: Hell SW]]
-[[Category: Schaefer, L.]]
+[[Category: Jakobs S]]
-[[Category: Stiel, A C.]]
+[[Category: Schaefer L]]
-[[Category: Trowitzsch, S.]]
+[[Category: Stiel AC]]
-[[Category: Wahl, M C.]]
+[[Category: Trowitzsch S]]
-[[Category: Weber, G.]]
+[[Category: Wahl MC]]
-[[Category: Ascp]]
+[[Category: Weber G]]
-[[Category: Chromoprotein]]
-[[Category: Fluorescent protein]]
-[[Category: Luminescent protein]]
-[[Category: Photochromic protein]]
-[[Category: Reversible photoswitch]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  1 09:47:52 2009''