1yk9: Difference between revisions

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-[[Image:1yk9.png|left|200px]]
-<!--
+==Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c==
-The line below this paragraph, containing "STRUCTURE_1yk9", creates the "Structure Box" on the page.
+<StructureSection load='1yk9' size='340' side='right'caption='[[1yk9]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1yk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YK9 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yk9 OCA], [https://pdbe.org/1yk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yk9 RCSB], [https://www.ebi.ac.uk/pdbsum/1yk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yk9 ProSAT]</span></td></tr>
-{{STRUCTURE_1yk9|  PDB=1yk9  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CYA1_MYCTU CYA1_MYCTU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yk/1yk9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yk9 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Rv1625c Class III adenylyl cyclase from Mycobacterium tuberculosis is a homodimeric enzyme with two catalytic centers at the dimer interface, and shows sequence similarity with the mammalian adenylyl and guanylyl cyclases. Mutation of the substrate-specifying residues in the catalytic domain of Rv1625c, either independently or together, to those present in guanylyl cyclases not only failed to confer guanylyl cyclase activity to the protein, but also severely abrogated the adenylyl cyclase activity of the enzyme. Biochemical analysis revealed alterations in the behavior of the mutants on ion-exchange chromatography, indicating differences in the surface-exposed charge upon mutation of substrate-specifying residues. The mutant proteins showed alterations in oligomeric status as compared to the wild-type enzyme, and differing abilities to heterodimerize with the wild-type protein. The crystal structure of a mutant has been solved to a resolution of 2.7A. On the basis of the structure, and additional biochemical studies, we provide possible reasons for the altered properties of the mutant proteins, as well as highlight unique structural features of the Rv1625c adenylyl cyclase.
-===Crystal structure of a mutant form of the mycobacterial adenylyl cyclase Rv1625c===
+A structural basis for the role of nucleotide specifying residues in regulating the oligomerization of the Rv1625c adenylyl cyclase from M. tuberculosis.,Ketkar AD, Shenoy AR, Ramagopal UA, Visweswariah SS, Suguna K J Mol Biol. 2006 Mar 3;356(4):904-16. Epub 2005 Dec 22. PMID:16403515<ref>PMID:16403515</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_16403515}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1yk9" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 16403515 is the PubMed ID number.
--->
-{{ABSTRACT_PUBMED_16403515}}
-==About this Structure==
-[[1yk9]] is a 1 chain structure of [[Adenylyl cyclase]] with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YK9 OCA].
 ==See Also==
-*[[Adenylyl cyclase]]
+*[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:16403515</ref><references group="xtra"/>
+__TOC__
-[[Category: Adenylate cyclase]]
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mycobacterium tuberculosis]]
-[[Category: Ketkar, A D.]]
+[[Category: Ketkar AD]]
-[[Category: Ramagopal, U A.]]
+[[Category: Ramagopal UA]]
-[[Category: Shenoy, A R.]]
+[[Category: Shenoy AR]]
-[[Category: Suguna, K.]]
+[[Category: Suguna K]]
-[[Category: TBSGC, TB Structural Genomics Consortium.]]
+[[Category: Visweswariah SS]]
-[[Category: Visweswariah, S S.]]
-[[Category: Beta-alpha-beta sandwich]]
-[[Category: Lyase]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Structural genomic]]
-[[Category: Tb structural genomics consortium]]
-[[Category: Tbsgc]]