2mzv: Difference between revisions
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==Resonance assignments and secondary structure of a phytocystatin from Sesamum indicum== | |||
<StructureSection load='2mzv' size='340' side='right'caption='[[2mzv]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mzv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sesamum_indicum Sesamum indicum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MZV FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mzv OCA], [https://pdbe.org/2mzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mzv RCSB], [https://www.ebi.ac.uk/pdbsum/2mzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mzv ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9AUC9_SESIN Q9AUC9_SESIN] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A cDNA encoding a cysteine protease inhibitor, cystatin was cloned from sesame (Sesamum indicum L.) seed. This clone was constructed into an expression vector and expressed in E. coli and purified to homogeneous. The recombinant sesame cystatin (SiCYS) showed effectively inhibitory activity toward C1 cysteine proteases. In order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear NMR techniques were used to characterize the structure of SiCYS. The full (1)H, (15)N, and (13)C resonances of SiCYS were assigned. The secondary structure of SiCYS was identified by using the assigned chemical shifts of (1)H(alpha), (13)C(alpha), (13)C(beta), and (13)CO through the consensus chemical shift index (CSI). The results of CSI analysis of SiCYS suggest eight beta-strands (residues 33-46, 51-61, 63-75, 80-87, 150-155, 157-169, 172-183, and 192-195) and two alpha-helices (residues 16-30, and 120-135). | |||
Resonance assignments and secondary structure of a phytocystatin from Sesamum indicum.,Hu YJ, Irene D, Lo CJ, Cai YL, Tzen TC, Lin TH, Chyan CL Biomol NMR Assign. 2015 Oct;9(2):309-11. doi: 10.1007/s12104-015-9598-y. Epub, 2015 Feb 12. PMID:25673506<ref>PMID:25673506</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Chyan | <div class="pdbe-citations 2mzv" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Sesamum indicum]] | |||
[[Category: Chyan C]] | |||
Latest revision as of 09:12, 15 May 2024
Resonance assignments and secondary structure of a phytocystatin from Sesamum indicumResonance assignments and secondary structure of a phytocystatin from Sesamum indicum
Structural highlights
FunctionPublication Abstract from PubMedA cDNA encoding a cysteine protease inhibitor, cystatin was cloned from sesame (Sesamum indicum L.) seed. This clone was constructed into an expression vector and expressed in E. coli and purified to homogeneous. The recombinant sesame cystatin (SiCYS) showed effectively inhibitory activity toward C1 cysteine proteases. In order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear NMR techniques were used to characterize the structure of SiCYS. The full (1)H, (15)N, and (13)C resonances of SiCYS were assigned. The secondary structure of SiCYS was identified by using the assigned chemical shifts of (1)H(alpha), (13)C(alpha), (13)C(beta), and (13)CO through the consensus chemical shift index (CSI). The results of CSI analysis of SiCYS suggest eight beta-strands (residues 33-46, 51-61, 63-75, 80-87, 150-155, 157-169, 172-183, and 192-195) and two alpha-helices (residues 16-30, and 120-135). Resonance assignments and secondary structure of a phytocystatin from Sesamum indicum.,Hu YJ, Irene D, Lo CJ, Cai YL, Tzen TC, Lin TH, Chyan CL Biomol NMR Assign. 2015 Oct;9(2):309-11. doi: 10.1007/s12104-015-9598-y. Epub, 2015 Feb 12. PMID:25673506[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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