2mvf: Difference between revisions
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==Structural insight into an essential assembly factor network on the pre-ribosome== | |||
<StructureSection load='2mvf' size='340' side='right'caption='[[2mvf]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MVF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mvf OCA], [https://pdbe.org/2mvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mvf RCSB], [https://www.ebi.ac.uk/pdbsum/2mvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mvf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G0S081_CHATD G0S081_CHATD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Eukaryotic ribosome biogenesis involves approximately 200 assembly factors, but how these contribute to ribosome maturation is poorly understood. Here, we identify a network of factors on the nascent 60S subunit that actively remodels preribosome structure. At its hub is Rsa4, a direct substrate of the force-generating ATPase Rea1. We show that Rsa4 is connected to the central protuberance by binding to Rpl5 and to ribosomal RNA (rRNA) helix 89 of the nascent peptidyl transferase center (PTC) through Nsa2. Importantly, Nsa2 binds to helix 89 before relocation of helix 89 to the PTC. Structure-based mutations of these factors reveal the functional importance of their interactions for ribosome assembly. Thus, Rsa4 is held tightly in the preribosome and can serve as a "distribution box," transmitting remodeling energy from Rea1 into the developing ribosome. We suggest that a relay-like factor network coupled to a mechano-enzyme is strategically positioned to relocate rRNA elements during ribosome maturation. | |||
A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation.,Bassler J, Paternoga H, Holdermann I, Thoms M, Granneman S, Barrio-Garcia C, Nyarko A, Stier G, Clark SA, Schraivogel D, Kallas M, Beckmann R, Tollervey D, Barbar E, Sinning I, Hurt E J Cell Biol. 2014 Nov 24;207(4):481-98. doi: 10.1083/jcb.201408111. Epub 2014 Nov, 17. PMID:25404745<ref>PMID:25404745</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2mvf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] | |||
[[Category: Large Structures]] | |||
[[Category: Barbar E]] | |||
[[Category: Barrio-Garcia C]] | |||
[[Category: Bassler J]] | |||
[[Category: Beckmann R]] | |||
[[Category: Clark SA]] | |||
[[Category: Granneman S]] | |||
[[Category: Holdermann I]] | |||
[[Category: Hurt E]] | |||
[[Category: Kallas M]] | |||
[[Category: Lee W]] | |||
[[Category: Nyarko A]] | |||
[[Category: Paternoga H]] | |||
[[Category: Schraivogel D]] | |||
[[Category: Sinning I]] | |||
[[Category: Stier G]] | |||
[[Category: Thomas M]] | |||
[[Category: Tollervey D]] | |||
Latest revision as of 09:10, 15 May 2024
Structural insight into an essential assembly factor network on the pre-ribosomeStructural insight into an essential assembly factor network on the pre-ribosome
Structural highlights
FunctionPublication Abstract from PubMedEukaryotic ribosome biogenesis involves approximately 200 assembly factors, but how these contribute to ribosome maturation is poorly understood. Here, we identify a network of factors on the nascent 60S subunit that actively remodels preribosome structure. At its hub is Rsa4, a direct substrate of the force-generating ATPase Rea1. We show that Rsa4 is connected to the central protuberance by binding to Rpl5 and to ribosomal RNA (rRNA) helix 89 of the nascent peptidyl transferase center (PTC) through Nsa2. Importantly, Nsa2 binds to helix 89 before relocation of helix 89 to the PTC. Structure-based mutations of these factors reveal the functional importance of their interactions for ribosome assembly. Thus, Rsa4 is held tightly in the preribosome and can serve as a "distribution box," transmitting remodeling energy from Rea1 into the developing ribosome. We suggest that a relay-like factor network coupled to a mechano-enzyme is strategically positioned to relocate rRNA elements during ribosome maturation. A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation.,Bassler J, Paternoga H, Holdermann I, Thoms M, Granneman S, Barrio-Garcia C, Nyarko A, Stier G, Clark SA, Schraivogel D, Kallas M, Beckmann R, Tollervey D, Barbar E, Sinning I, Hurt E J Cell Biol. 2014 Nov 24;207(4):481-98. doi: 10.1083/jcb.201408111. Epub 2014 Nov, 17. PMID:25404745[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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