2mow: Difference between revisions

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'''Unreleased structure'''


The entry 2mow is ON HOLD
==Structure of Nrd1p CID - Trf4p NIM complex==
<StructureSection load='2mow' size='340' side='right'caption='[[2mow]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2mow]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MOW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mow OCA], [https://pdbe.org/2mow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mow RCSB], [https://www.ebi.ac.uk/pdbsum/2mow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mow ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NRD1_YEAST NRD1_YEAST] Plays a role in sequence-specific regulation of nuclear pre-mRNA abundance.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. We propose that transcription termination and RNA degradation are coordinated by switching between two alternative partners of the NNS complex.


Authors: Kabzinski, T., Stefl, R., Kubicek, K.
Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation.,Tudek A, Porrua O, Kabzinski T, Lidschreiber M, Kubicek K, Fortova A, Lacroute F, Vanacova S, Cramer P, Stefl R, Libri D Mol Cell. 2014 Aug 7;55(3):467-481. doi: 10.1016/j.molcel.2014.05.031. Epub 2014 , Jul 24. PMID:25066235<ref>PMID:25066235</ref>


Description: Structure of Nrd1p CID -Trf4p NIM complex
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2mow" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Kabzinski T]]
[[Category: Kubicek K]]
[[Category: Stefl R]]

Latest revision as of 09:08, 15 May 2024

Structure of Nrd1p CID - Trf4p NIM complexStructure of Nrd1p CID - Trf4p NIM complex

Structural highlights

2mow is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NRD1_YEAST Plays a role in sequence-specific regulation of nuclear pre-mRNA abundance.

Publication Abstract from PubMed

The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. We propose that transcription termination and RNA degradation are coordinated by switching between two alternative partners of the NNS complex.

Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation.,Tudek A, Porrua O, Kabzinski T, Lidschreiber M, Kubicek K, Fortova A, Lacroute F, Vanacova S, Cramer P, Stefl R, Libri D Mol Cell. 2014 Aug 7;55(3):467-481. doi: 10.1016/j.molcel.2014.05.031. Epub 2014 , Jul 24. PMID:25066235[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Tudek A, Porrua O, Kabzinski T, Lidschreiber M, Kubicek K, Fortova A, Lacroute F, Vanacova S, Cramer P, Stefl R, Libri D. Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation. Mol Cell. 2014 Aug 7;55(3):467-481. doi: 10.1016/j.molcel.2014.05.031. Epub 2014 , Jul 24. PMID:25066235 doi:http://dx.doi.org/10.1016/j.molcel.2014.05.031
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