2m4v: Difference between revisions
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== | ==Mycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factors== | ||
[[2m4v]] is a 1 chain structure with sequence from [ | <StructureSection load='2m4v' size='340' side='right'caption='[[2m4v]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2m4v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M4V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m4v OCA], [https://pdbe.org/2m4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m4v RCSB], [https://www.ebi.ac.uk/pdbsum/2m4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m4v ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA polymerase binding protein A (RbpA), encoded by Rv2050, is specific to the actinomycetes, where it is highly conserved. In the pathogen Mycobacterium tuberculosis, RbpA is essential for growth and survival. RbpA binds to the beta subunit of the RNA polymerase where it activates transcription by unknown mechanisms, and it may also influence the response of M. tuberculosis to the current frontline anti-tuberculosis drug rifampicin. Here we report the solution structure of RbpA and identify the principle sigma factor sigmaA, and the stress-induced sigmaB, as interaction partners. The protein has a central ordered domain with a conserved hydrophobic surface that may be a potential protein interaction site. The N- and C- termini are highly dynamic and are involved in the interaction with the sigma factors. RbpA forms a tight complex with the N-terminal domain of sigmaB via its N- and C-terminal regions. The interaction with sigma factors may explain how RbpA stabilises sigma subunit binding to the core RNA polymerase and thereby promotes initiation complex formation. RbpA could therefore influence the competition between principal and alternative sigma factors and hence the transcription profile of the cell. | |||
Mycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factors.,Bortoluzzi A, Muskett FW, Waters LC, Addis PW, Rieck B, Munder T, Schleier S, Forti F, Ghisotti D, Carr MD, O'Hare HM J Biol Chem. 2013 Apr 10. PMID:23548911<ref>PMID:23548911</ref> | |||
<ref | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2m4v" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
[[Category: Addis | [[Category: Addis PW]] | ||
[[Category: Bortoluzzi | [[Category: Bortoluzzi A]] | ||
[[Category: Carr | [[Category: Carr MD]] | ||
[[Category: Forti | [[Category: Forti F]] | ||
[[Category: Ghisotti | [[Category: Ghisotti D]] | ||
[[Category: | [[Category: Munder T]] | ||
[[Category: | [[Category: Muskett FW]] | ||
[[Category: | [[Category: O'Hare HM]] | ||
[[Category: Rieck | [[Category: Rieck B]] | ||
[[Category: Schleier | [[Category: Schleier S]] | ||
[[Category: Waters | [[Category: Waters LC]] | ||
Latest revision as of 08:59, 15 May 2024
Mycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factorsMycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factors
Structural highlights
Publication Abstract from PubMedRNA polymerase binding protein A (RbpA), encoded by Rv2050, is specific to the actinomycetes, where it is highly conserved. In the pathogen Mycobacterium tuberculosis, RbpA is essential for growth and survival. RbpA binds to the beta subunit of the RNA polymerase where it activates transcription by unknown mechanisms, and it may also influence the response of M. tuberculosis to the current frontline anti-tuberculosis drug rifampicin. Here we report the solution structure of RbpA and identify the principle sigma factor sigmaA, and the stress-induced sigmaB, as interaction partners. The protein has a central ordered domain with a conserved hydrophobic surface that may be a potential protein interaction site. The N- and C- termini are highly dynamic and are involved in the interaction with the sigma factors. RbpA forms a tight complex with the N-terminal domain of sigmaB via its N- and C-terminal regions. The interaction with sigma factors may explain how RbpA stabilises sigma subunit binding to the core RNA polymerase and thereby promotes initiation complex formation. RbpA could therefore influence the competition between principal and alternative sigma factors and hence the transcription profile of the cell. Mycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factors.,Bortoluzzi A, Muskett FW, Waters LC, Addis PW, Rieck B, Munder T, Schleier S, Forti F, Ghisotti D, Carr MD, O'Hare HM J Biol Chem. 2013 Apr 10. PMID:23548911[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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