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==Solution structure of chicken ileal BABP in complex with glycochenodeoxycholic acid== | ==Solution structure of chicken ileal BABP in complex with glycochenodeoxycholic acid== | ||
<StructureSection load='2lba' size='340' side='right' caption='[[2lba | <StructureSection load='2lba' size='340' side='right'caption='[[2lba]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
[[2lba]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[2lba]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LBA FirstGlance]. <br> | ||
<b>[[Ligand|Ligands:]]</b> <scene name='pdbligand=CHO:GLYCOCHENODEOXYCHOLIC+ACID'>CHO</scene>< | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHO:GLYCOCHENODEOXYCHOLIC+ACID'>CHO</scene></td></tr> | |||
<b>Resources:</b> <span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lba OCA], [https://pdbe.org/2lba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lba RCSB], [https://www.ebi.ac.uk/pdbsum/2lba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lba ProSAT]</span></td></tr> | ||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/F1NUJ7_CHICK F1NUJ7_CHICK] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Ileal bile acid binding proteins (I-BABP), belonging to the family of intracellular lipid binding proteins, control bile acid trafficking in enterocytes and participate in regulating the homeostasis of these cholesterol-derived metabolites. I-BABP orthologues share the same structural fold and are able to host up to two ligands in their large internal cavity. However variations in primary sequences determine differences in binding properties such as the degree of binding cooperativity. To investigate the molecular requirements for cooperativity we adopted a gain-of-function approach, exploring the possibility to turn the non-cooperative chicken I-BABP (cI-BABP) into a cooperative mutant protein. To this aim we first solved the solution structure of cI-BABP in complex with two molecules of the physiological ligand glycochenodeoxycholate. A comparative structural analysis with closely related members of the same protein family provided the basis to design a double mutant (H99Q/A101S cI-BABP) capable of establishing a cooperative binding mechanism. Molecular dynamics simulation studies of the wild type and mutant complexes and essential dynamics analysis of the trajectories supported the role of the identified amino acid residues as hot spot mediators of communication between binding sites. The emerging picture is consistent with a binding mechanism that can be described by an extended conformational selection model. | Ileal bile acid binding proteins (I-BABP), belonging to the family of intracellular lipid binding proteins, control bile acid trafficking in enterocytes and participate in regulating the homeostasis of these cholesterol-derived metabolites. I-BABP orthologues share the same structural fold and are able to host up to two ligands in their large internal cavity. However variations in primary sequences determine differences in binding properties such as the degree of binding cooperativity. To investigate the molecular requirements for cooperativity we adopted a gain-of-function approach, exploring the possibility to turn the non-cooperative chicken I-BABP (cI-BABP) into a cooperative mutant protein. To this aim we first solved the solution structure of cI-BABP in complex with two molecules of the physiological ligand glycochenodeoxycholate. A comparative structural analysis with closely related members of the same protein family provided the basis to design a double mutant (H99Q/A101S cI-BABP) capable of establishing a cooperative binding mechanism. Molecular dynamics simulation studies of the wild type and mutant complexes and essential dynamics analysis of the trajectories supported the role of the identified amino acid residues as hot spot mediators of communication between binding sites. The emerging picture is consistent with a binding mechanism that can be described by an extended conformational selection model. | ||
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Structural requirements for cooperativity in ileal bile acid binding proteins.,Zanzoni S, Assfalg M, Giorgetti A, D'Onofrio M, Molinari H J Biol Chem. 2011 Sep 14. PMID:21917914<ref>PMID:21917914</ref> | Structural requirements for cooperativity in ileal bile acid binding proteins.,Zanzoni S, Assfalg M, Giorgetti A, D'Onofrio M, Molinari H J Biol Chem. 2011 Sep 14. PMID:21917914<ref>PMID:21917914</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
<div class="pdbe-citations 2lba" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
| Line 17: | Line 24: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Assfalg M]] | ||
[[Category: D'Onofrio M]] | |||
[[Category: Onofrio | [[Category: Giorgetti A]] | ||
[[Category: | [[Category: Molinari H]] | ||
[[Category: | [[Category: Zanzoni S]] | ||
[[Category: | |||
Latest revision as of 08:40, 15 May 2024
Solution structure of chicken ileal BABP in complex with glycochenodeoxycholic acidSolution structure of chicken ileal BABP in complex with glycochenodeoxycholic acid
Structural highlights
FunctionPublication Abstract from PubMedIleal bile acid binding proteins (I-BABP), belonging to the family of intracellular lipid binding proteins, control bile acid trafficking in enterocytes and participate in regulating the homeostasis of these cholesterol-derived metabolites. I-BABP orthologues share the same structural fold and are able to host up to two ligands in their large internal cavity. However variations in primary sequences determine differences in binding properties such as the degree of binding cooperativity. To investigate the molecular requirements for cooperativity we adopted a gain-of-function approach, exploring the possibility to turn the non-cooperative chicken I-BABP (cI-BABP) into a cooperative mutant protein. To this aim we first solved the solution structure of cI-BABP in complex with two molecules of the physiological ligand glycochenodeoxycholate. A comparative structural analysis with closely related members of the same protein family provided the basis to design a double mutant (H99Q/A101S cI-BABP) capable of establishing a cooperative binding mechanism. Molecular dynamics simulation studies of the wild type and mutant complexes and essential dynamics analysis of the trajectories supported the role of the identified amino acid residues as hot spot mediators of communication between binding sites. The emerging picture is consistent with a binding mechanism that can be described by an extended conformational selection model. Structural requirements for cooperativity in ileal bile acid binding proteins.,Zanzoni S, Assfalg M, Giorgetti A, D'Onofrio M, Molinari H J Biol Chem. 2011 Sep 14. PMID:21917914[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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