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The entry | ==Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A== | ||
<StructureSection load='2l6u' size='340' side='right'caption='[[2l6u]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2l6u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L6U FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l6u OCA], [https://pdbe.org/2l6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l6u RCSB], [https://www.ebi.ac.uk/pdbsum/2l6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l6u ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[https://www.uniprot.org/uniprot/MED25_HUMAN MED25_HUMAN] Charcot-Marie-Tooth disease type 2B2. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:19290556</ref> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MED25_HUMAN MED25_HUMAN] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription.<ref>PMID:14657022</ref> <ref>PMID:14983011</ref> <ref>PMID:17641689</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The solution NMR structure of protein MED25(391-543), comprising the activator interacting domain (ACID) of subunit 25 of the human mediator, is presented along with the measurement of polypeptide backbone heteronuclear (15)N-{(1)H} NOEs to identify fast internal motional modes. This domain interacts with the acidic transactivation domains of Herpes simplex type 1 (HSV-1) protein VP16 and the Varicella-zoster virus (VZV) major transactivator protein IE62, which initiate transcription of viral genes. The structure is similar to the beta-barrel domains of the human protein Ku and the SPOC domain of human protein SHARP, and provides a starting point to understand the structural biology of initiation of HSV-1 and VZV gene activation. Homology models built for the two ACID domains of the prostate tumor overexpressed (PTOV1) protein using the structure of MED25(391-543) as a template suggest that differential biological activities of the ACID domains in MED25 and PTOV1 arise from modulation of quite similar protein-protein interactions by variable residues grouped around highly conserved charged surface areas. | |||
Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25.,Eletsky A, Ruyechan WT, Xiao R, Acton TB, Montelione GT, Szyperski T J Struct Funct Genomics. 2011 Sep;12(3):159-66. Epub 2011 Jul 23. PMID:21785987<ref>PMID:21785987</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2l6u" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Mediator|Mediator]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Acton TB]] | |||
[[Category: Ciccosanti C]] | |||
[[Category: Eletsky A]] | |||
[[Category: Everett JK]] | |||
[[Category: Janjua H]] | |||
[[Category: Montelione GT]] | |||
[[Category: Ryuechan WT]] | |||
[[Category: Shastry R]] | |||
[[Category: Sukumaran DK]] | |||
[[Category: Szyperski T]] | |||
[[Category: Xiao R]] | |||