1wj2: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Solution Structure of the C-terminal WRKY Domain of AtWRKY4== | ==Solution Structure of the C-terminal WRKY Domain of AtWRKY4== | ||
<StructureSection load='1wj2' size='340' side='right'caption='[[1wj2 | <StructureSection load='1wj2' size='340' side='right'caption='[[1wj2]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1wj2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[1wj2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WJ2 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wj2 OCA], [https://pdbe.org/1wj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wj2 RCSB], [https://www.ebi.ac.uk/pdbsum/1wj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wj2 ProSAT], [https://www.topsan.org/Proteins/RSGI/1wj2 TOPSAN]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wj2 OCA], [https://pdbe.org/1wj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wj2 RCSB], [https://www.ebi.ac.uk/pdbsum/1wj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wj2 ProSAT], [https://www.topsan.org/Proteins/RSGI/1wj2 TOPSAN]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/WRKY4_ARATH WRKY4_ARATH] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 33: | Line 33: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Arabidopsis thaliana]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Inoue | [[Category: Inoue M]] | ||
[[Category: Kigawa | [[Category: Kigawa T]] | ||
[[Category: Yamasaki K]] | |||
[[Category: Yamasaki | [[Category: Yokoyama S]] | ||
[[Category: Yokoyama | |||
Latest revision as of 16:42, 9 May 2024
Solution Structure of the C-terminal WRKY Domain of AtWRKY4Solution Structure of the C-terminal WRKY Domain of AtWRKY4
Structural highlights
FunctionWRKY4_ARATH Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe WRKY proteins comprise a major family of transcription factors that are essential in pathogen and salicylic acid responses of higher plants as well as a variety of plant-specific reactions. They share a DNA binding domain, designated as the WRKY domain, which contains an invariant WRKYGQK sequence and a CX4-5CX22-23HXH zinc binding motif. Herein, we report the NMR solution structure of the C-terminal WRKY domain of the Arabidopsis thaliana WRKY4 protein. The structure consists of a four-stranded beta-sheet, with a zinc binding pocket formed by the conserved Cys/His residues located at one end of the beta-sheet, revealing a novel zinc and DNA binding structure. The WRKYGQK residues correspond to the most N-terminal beta-strand, kinked in the middle of the sequence by the Gly residue, which enables extensive hydrophobic interactions involving the Trp residue and contributes to the structural stability of the beta-sheet. Based on a profile of NMR chemical shift perturbations, we propose that the same strand enters the DNA groove and forms contacts with the DNA bases. Solution structure of an Arabidopsis WRKY DNA binding domain.,Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Hayami N, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S Plant Cell. 2005 Mar;17(3):944-56. Epub 2005 Feb 10. PMID:15705956[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||