5coj: Difference between revisions

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-'''Unreleased structure'''
-The entry 5coj is ON HOLD
+==Structure of Hydroxyethylthiazole kinase ThiM from Staphylococcus aureus in complex with native substrate 2-(4-methyl-1,3-thiazol-5-yl)ethanol.==
+<StructureSection load='5coj' size='340' side='right'caption='[[5coj]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5coj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5COJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5COJ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TZE:2-(4-METHYL-THIAZOL-5-YL)-ETHANOL'>TZE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5coj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5coj OCA], [https://pdbe.org/5coj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5coj RCSB], [https://www.ebi.ac.uk/pdbsum/5coj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5coj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/THIM_STAAR THIM_STAAR] Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Infections caused by the methicillin-resistant Staphylococcus aureus (MRSA) are today known to be a substantial threat for global health. Emerging multi-drug resistant bacteria have created a substantial need to identify and discover new drug targets and to develop novel strategies to treat bacterial infections. A promising and so far untapped antibiotic target is the biosynthesis of vitamin B1 (thiamin). Thiamin in its activated form, thiamin pyrophosphate, is an essential co-factor for all organisms. Therefore, thiamin analogous compounds, when introduced into the vitamin B1 biosynthetic pathway and further converted into non-functional co-factors by the bacterium can function as pro-drugs which thus block various co-factor dependent pathways. We characterized one of the key enzymes within the S. aureus vitamin B1 biosynthetic pathway, 5-(hydroxyethyl)-4-methylthiazole kinase (SaThiM; EC 2.7.1.50), a potential target for pro-drug compounds and analyzed the native structure of SaThiM and complexes with the natural substrate 5-(hydroxyethyl)-4-methylthiazole (THZ) and two selected substrate analogues.
-Authors: Drebes, J., Kuenz, M., Eberle, R.J., Oberthuer, D., Cang, H., Wrenger, C., Betzel, C.
+Structure of ThiM from Vitamin B1 biosynthetic pathway of Staphylococcus aureus - Insights into a novel pro-drug approach addressing MRSA infections.,Drebes J, Kunz M, Windshugel B, Kikhney AG, Muller IB, Eberle RJ, Oberthur D, Cang H, Svergun DI, Perbandt M, Betzel C, Wrenger C Sci Rep. 2016 Mar 10;6:22871. doi: 10.1038/srep22871. PMID:26960569<ref>PMID:26960569</ref>
-Description: Structure of Hydroxyethylthiazole kinase ThiM from Staphylococcus aureus in complex with native substrate 2-(4-methyl-1,3-thiazol-5-yl)ethanol.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Kuenz, M]]
+<div class="pdbe-citations 5coj" style="background-color:#fffaf0;"></div>
-[[Category: Oberthuer, D]]
+== References ==
-[[Category: Wrenger, C]]
+<references/>
-[[Category: Eberle, R.J]]
+__TOC__
-[[Category: Cang, H]]
+</StructureSection>
-[[Category: Drebes, J]]
+[[Category: Large Structures]]
-[[Category: Betzel, C]]
+[[Category: Staphylococcus aureus]]
+[[Category: Betzel C]]
+[[Category: Cang H]]
+[[Category: Drebes J]]
+[[Category: Eberle RJ]]
+[[Category: Kuenz M]]
+[[Category: Oberthuer D]]
+[[Category: Wrenger C]]