5a2o: Difference between revisions

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==Crystal structure of the nitrate transporter NRT1.1 from Arabidopsis thaliana in complex with nitrate.==
==Crystal structure of the nitrate transporter NRT1.1 from Arabidopsis thaliana in complex with nitrate.==
<StructureSection load='5a2o' size='340' side='right' caption='[[5a2o]], [[Resolution|resolution]] 3.71&Aring;' scene=''>
<StructureSection load='5a2o' size='340' side='right'caption='[[5a2o]], [[Resolution|resolution]] 3.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5a2o]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4cl5 4cl5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A2O FirstGlance]. <br>
<table><tr><td colspan='2'>[[5a2o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4cl5 4cl5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A2O FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.71&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a2n|5a2n]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2o OCA], [http://pdbe.org/5a2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a2o RCSB], [http://www.ebi.ac.uk/pdbsum/5a2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a2o ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a2o OCA], [https://pdbe.org/5a2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a2o RCSB], [https://www.ebi.ac.uk/pdbsum/5a2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a2o ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PTR7_ARATH PTR7_ARATH]] Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation.<ref>PMID:8453665</ref> <ref>PMID:9844028</ref> <ref>PMID:12606566</ref> <ref>PMID:12509525</ref> <ref>PMID:15319483</ref> <ref>PMID:17148611</ref> <ref>PMID:19766570</ref> <ref>PMID:19633234</ref> <ref>PMID:20627075</ref>
[https://www.uniprot.org/uniprot/PTR7_ARATH PTR7_ARATH] Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation.<ref>PMID:8453665</ref> <ref>PMID:9844028</ref> <ref>PMID:12606566</ref> <ref>PMID:12509525</ref> <ref>PMID:15319483</ref> <ref>PMID:17148611</ref> <ref>PMID:19766570</ref> <ref>PMID:19633234</ref> <ref>PMID:20627075</ref>  
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Newstead, S]]
[[Category: Arabidopsis thaliana]]
[[Category: Parker, J L]]
[[Category: Large Structures]]
[[Category: Mf]]
[[Category: Newstead S]]
[[Category: Mfs transporter]]
[[Category: Parker JL]]
[[Category: Nitrate]]
[[Category: Npf family]]
[[Category: Nrt1/ptr family]]
[[Category: Pot family]]
[[Category: Transport protein]]
[[Category: Transporter]]

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