4ycr: Difference between revisions
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The | ==Structure determination of an integral membrane protein at room temperature from crystals in situ== | ||
<StructureSection load='4ycr' size='340' side='right'caption='[[4ycr]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ycr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YCR FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ycr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ycr OCA], [https://pdbe.org/4ycr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ycr RCSB], [https://www.ebi.ac.uk/pdbsum/4ycr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ycr ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TEHA_HAEIN TEHA_HAEIN] Ion channel involved in potassium tellurite resistance. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 A resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines. | |||
Structure determination of an integral membrane protein at room temperature from crystals in situ.,Axford D, Foadi J, Hu NJ, Choudhury HG, Iwata S, Beis K, Evans G, Alguel Y Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1228-37. doi:, 10.1107/S139900471500423X. Epub 2015 May 14. PMID:26057664<ref>PMID:26057664</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4ycr" style="background-color:#fffaf0;"></div> | ||
[[Category: Alguel | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Evans | </StructureSection> | ||
[[Category: | [[Category: Haemophilus influenzae Rd KW20]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Alguel Y]] | ||
[[Category: Axford D]] | |||
[[Category: Beis K]] | |||
[[Category: Choudhury HG]] | |||
[[Category: Evans G]] | |||
[[Category: Foadi J]] | |||
[[Category: Hu NJ]] | |||
[[Category: Iwata S]] | |||
Latest revision as of 14:31, 9 May 2024
Structure determination of an integral membrane protein at room temperature from crystals in situStructure determination of an integral membrane protein at room temperature from crystals in situ
Structural highlights
FunctionTEHA_HAEIN Ion channel involved in potassium tellurite resistance. Publication Abstract from PubMedThe structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 A resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines. Structure determination of an integral membrane protein at room temperature from crystals in situ.,Axford D, Foadi J, Hu NJ, Choudhury HG, Iwata S, Beis K, Evans G, Alguel Y Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1228-37. doi:, 10.1107/S139900471500423X. Epub 2015 May 14. PMID:26057664[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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