4atu: Difference between revisions

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-[[Image:4atu.jpg|left|200px]]
-{{STRUCTURE_4atu|  PDB=4atu  |  SCENE=  }}
+==Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin==
+<SX load='4atu' size='340' side='right' viewer='molstar' caption='[[4atu]], [[Resolution|resolution]] 8.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4atu]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4atu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atu OCA], [https://pdbe.org/4atu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4atu RCSB], [https://www.ebi.ac.uk/pdbsum/4atu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4atu ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Doublecortin (Dcx) defines a growing family of microtubule (MT)-associated proteins (MAPs) involved in neuronal migration and process outgrowth. We show that Dcx is essential for the function of Kif1a, a kinesin-3 motor protein that traffics synaptic vesicles. Neurons lacking Dcx and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-mediated transport of Vamp2, a cargo of Kif1a, with decreased run length. Human disease-associated mutations in Dcx's linker sequence (e.g., W146C, K174E) alter Kif1a/Vamp2 transport by disrupting Dcx/Kif1a interactions without affecting Dcx MT binding. Dcx specifically enhances binding of the ADP-bound Kif1a motor domain to MTs. Cryo-electron microscopy and subnanometer-resolution image reconstruction reveal the kinesin-dependent conformational variability of MT-bound Dcx and suggest a model for MAP-motor crosstalk on MTs. Alteration of kinesin run length by MAPs represents a previously undiscovered mode of control of kinesin transport and provides a mechanism for regulation of MT-based transport by local signals.
-===Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin===
+Molecular basis for specific regulation of neuronal Kinesin-3 motors by doublecortin family proteins.,Liu JS, Schubert CR, Fu X, Fourniol FJ, Jaiswal JK, Houdusse A, Stultz CM, Moores CA, Walsh CA Mol Cell. 2012 Sep 14;47(5):707-21. doi: 10.1016/j.molcel.2012.06.025. Epub 2012 , Aug 1. PMID:22857951<ref>PMID:22857951</ref>
-{{ABSTRACT_PUBMED_22857951}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4atu" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-[[4atu]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATU OCA].
+*[[Tubulin 3D Structures|Tubulin 3D Structures]]
+== References ==
+<references/>
+__TOC__
+</SX>
 [[Category: Bos taurus]]
 [[Category: Homo sapiens]]
-[[Category: Tubulin GTPase]]
+[[Category: Large Structures]]
-[[Category: Fourniol, F J.]]
+[[Category: Fourniol FJ]]
-[[Category: Fu, X.]]
+[[Category: Fu X]]
-[[Category: Houdusse, A.]]
+[[Category: Houdusse A]]
-[[Category: Jaiswal, J K.]]
+[[Category: Jaiswal JK]]
-[[Category: Liu, J S.]]
+[[Category: Liu JS]]
-[[Category: Moores, C A.]]
+[[Category: Moores CA]]
-[[Category: Schubert, C R.]]
+[[Category: Schubert CR]]
-[[Category: Stultz, C M.]]
+[[Category: Stultz CM]]
-[[Category: Walsh, C A.]]
+[[Category: Walsh CA]]
-[[Category: Hydrolase]]
-[[Category: Microtubule-associated protein]]