4abo: Difference between revisions
Jump to navigation
Jump to search
New page: '''Unreleased structure''' The entry 4abo is ON HOLD Authors: Maurer, S.P., Fourniol, F.J., Bohner, G., Moores, C.A., Surrey, T. Description: Mal3 CH domain homology model and mammalia... |
No edit summary |
||
| (11 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Mal3 CH domain homology model and mammalian tubulin (2XRP) docked into the 8.6-Angstrom cryo-EM map of Mal3-GTPgammaS-microtubules== | ||
<SX load='4abo' size='340' side='right' viewer='molstar' caption='[[4abo]], [[Resolution|resolution]] 8.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4abo]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ABO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ABO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4abo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4abo OCA], [https://pdbe.org/4abo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4abo RCSB], [https://www.ebi.ac.uk/pdbsum/4abo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4abo ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TBB_PIG TBB_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Growing microtubule ends serve as transient binding platforms for essential proteins that regulate microtubule dynamics and their interactions with cellular substructures. End-binding proteins (EBs) autonomously recognize an extended region at growing microtubule ends with unknown structural characteristics and then recruit other factors to the dynamic end structure. Using cryo-electron microscopy, subnanometer single-particle reconstruction, and fluorescence imaging, we present a pseudoatomic model of how the calponin homology (CH) domain of the fission yeast EB Mal3 binds to the end regions of growing microtubules. The Mal3 CH domain bridges protofilaments except at the microtubule seam. By binding close to the exchangeable GTP-binding site, the CH domain is ideally positioned to sense the microtubule's nucleotide state. The same microtubule-end region is also a stabilizing structural cap protecting the microtubule from depolymerization. This insight supports a common structural link between two important biological phenomena, microtubule dynamic instability and end tracking. | |||
EBs recognize a nucleotide-dependent structural cap at growing microtubule ends.,Maurer SP, Fourniol FJ, Bohner G, Moores CA, Surrey T Cell. 2012 Apr 13;149(2):371-82. PMID:22500803<ref>PMID:22500803</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4abo" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Schizosaccharomyces pombe]] | |||
[[Category: Sus scrofa]] | |||
[[Category: Bohner G]] | |||
[[Category: Fourniol FJ]] | |||
[[Category: Maurer SP]] | |||
[[Category: Moores CA]] | |||
[[Category: Surrey T]] | |||
Latest revision as of 13:49, 9 May 2024
Mal3 CH domain homology model and mammalian tubulin (2XRP) docked into the 8.6-Angstrom cryo-EM map of Mal3-GTPgammaS-microtubulesMal3 CH domain homology model and mammalian tubulin (2XRP) docked into the 8.6-Angstrom cryo-EM map of Mal3-GTPgammaS-microtubules
| |||||||||