2xmj: Difference between revisions

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-[[Image:2xmj.png|left|200px]]
-<!--
+==Visualising the Metal-binding Versatility of Copper Trafficking Sites: Atx1 side-to-side (aerobic)==
-The line below this paragraph, containing "STRUCTURE_2xmj", creates the "Structure Box" on the page.
+<StructureSection load='2xmj' size='340' side='right'caption='[[2xmj]], [[Resolution|resolution]] 1.08&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2xmj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XMJ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.08&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-{{STRUCTURE_2xmj|  PDB=2xmj  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xmj OCA], [https://pdbe.org/2xmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xmj RCSB], [https://www.ebi.ac.uk/pdbsum/2xmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xmj ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/P73213_SYNY3 P73213_SYNY3]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xm/2xmj_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xmj ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Molecular systems have evolved to permit the safe delivery of copper. Despite extensive studies, many copper site structures involved in copper homeostasis, even for the well-studied metallochaperone Atx1, remain unresolved. Cyanobacteria import copper to their thylakoid compartments for use in photosynthesis and respiration and possess an Atx1 that we show can adopt multiple oligomeric states when metalated, capable of binding up to four copper ions. Two-copper- and four-copper-loaded dimers exist in solution at low micromolar concentrations, and head-to-head and side-to-side arrangements, respectively, can be crystallized, with the latter binding a [Cu(4){mu(2)-S(gamma)(Cys)}(4)Cl(2)](2-) cluster. The His61Tyr mutation on loop 5 weakens head-to-head dimerization, yet a side-to-side dimer binding a similar cluster as in the wild-type protein, but with phenolate coordination, is present. The cognate metal-binding domains (MBDs) of the P-type ATPases CtaA and PacS, which are proposed to donate copper to and accept copper from Atx1, respectively, are monomeric in the presence of copper. The structure of the MBD of Cu(I)-PacS shows a crystallographic trimer arrangement around a [Cu(3){mu(2)-S(gamma)(Cys)}(3){S(gamma)(Cys)}(3)](2-) cluster that is very similar to that found for an alternate form of the His61Tyr Atx1 mutant. Copper transfer from the MBD of CtaA to Atx1 is favorable, but delivery from Atx1 to the MBD of PacS is strongly dependent upon the dimeric form of Atx1. A copper-induced switch in Atx1 dimer structure may have a regulatory role with cluster formation helping to buffer copper.
-===VISUALISING THE METAL-BINDING VERSATILITY OF COPPER TRAFFICKING SITES:ATX1 SIDE-TO-SIDE (AEROBIC)===
+Visualizing the metal-binding versatility of copper trafficking sites .,Badarau A, Firbank SJ, McCarthy AA, Banfield MJ, Dennison C Biochemistry. 2010 Sep 14;49(36):7798-810. PMID:20726513<ref>PMID:20726513</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-[[2xmj]] is a 2 chain structure of [[ABC transporter]] with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp._pcc_6803 Synechocystis sp. pcc 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XMJ OCA].
+<div class="pdbe-citations 2xmj" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[ABC transporter]]
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
-[[Category: Synechocystis sp. pcc 6803]]
+== References ==
-[[Category: Badarau, A.]]
+<references/>
-[[Category: Banfield, M J.]]
+__TOC__
-[[Category: Dennison, C.]]
+</StructureSection>
-[[Category: Firbank, S J.]]
+[[Category: Large Structures]]
-[[Category: Mccarthy, A A.]]
+[[Category: Synechocystis sp. PCC 6803]]
-[[Category: Chaperone]]
+[[Category: Badarau A]]
-[[Category: Copper homeostasis]]
+[[Category: Banfield MJ]]
-[[Category: Metal transport]]
+[[Category: Dennison C]]
-[[Category: P-type atpase]]
+[[Category: Firbank SJ]]
+[[Category: McCarthy AA]]