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<StructureSection load='2xdy' size='340' side='right'caption='[[2xdy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='2xdy' size='340' side='right'caption='[[2xdy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2xdy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XDY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XDY FirstGlance]. <br>
<table><tr><td colspan='2'>[[2xdy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XDY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XDY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xdy OCA], [http://pdbe.org/2xdy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xdy RCSB], [http://www.ebi.ac.uk/pdbsum/2xdy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xdy ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xdy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xdy OCA], [https://pdbe.org/2xdy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xdy RCSB], [https://www.ebi.ac.uk/pdbsum/2xdy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xdy ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9P8T1_NEUCS Q9P8T1_NEUCS]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chrysonilia crassa]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Boland, A]]
[[Category: Neurospora crassa]]
[[Category: Tritschler, F]]
[[Category: Boland A]]
[[Category: Weichenrieder, O]]
[[Category: Tritschler F]]
[[Category: 5' end binding]]
[[Category: Weichenrieder O]]
[[Category: Argonaute]]
[[Category: Gene silencing]]
[[Category: Rna binding protein]]
[[Category: Rna-induced silencing complex]]
[[Category: Rossmann-like fold]]

Latest revision as of 13:25, 9 May 2024

Crystal structure of the N. crassa QDE-2 AGO MID domainCrystal structure of the N. crassa QDE-2 AGO MID domain

Structural highlights

2xdy is a 1 chain structure with sequence from Neurospora crassa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9P8T1_NEUCS

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Argonaute (AGO) proteins are core components of RNA-induced silencing complexes and have essential roles in RNA-mediated gene silencing. They are characterized by a bilobal architecture, consisting of one lobe containing the amino-terminal and PAZ domains and another containing the MID and PIWI domains. Except for the PAZ domain, structural information on eukaryotic AGO domains is not yet available. In this study, we report the crystal structure of the MID domain of the eukaryotic AGO protein QDE-2 from Neurospora crassa. This domain adopts a Rossmann-like fold and recognizes the 5'-terminal nucleotide of a guide RNA in a manner similar to its prokaryotic counterparts. The 5'-nucleotide-binding site shares common residues with a second, adjacent ligand-binding site, suggesting a mechanism for the cooperative binding of ligands to the MID domain of eukaryotic AGOs.

Crystal structure and ligand binding of the MID domain of a eukaryotic Argonaute protein.,Boland A, Tritschler F, Heimstadt S, Izaurralde E, Weichenrieder O EMBO Rep. 2010 Jul;11(7):522-7. Epub 2010 Jun 11. PMID:20539312[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Boland A, Tritschler F, Heimstadt S, Izaurralde E, Weichenrieder O. Crystal structure and ligand binding of the MID domain of a eukaryotic Argonaute protein. EMBO Rep. 2010 Jul;11(7):522-7. Epub 2010 Jun 11. PMID:20539312 doi:10.1038/embor.2010.81

2xdy, resolution 2.20Å

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