2wdc: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| (13 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
< | ==Termus thermophilus Sulfate thiohydrolase SoxB in complex with glycerol== | ||
<StructureSection load='2wdc' size='340' side='right'caption='[[2wdc]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2wdc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WDC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wdc OCA], [https://pdbe.org/2wdc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wdc RCSB], [https://www.ebi.ac.uk/pdbsum/2wdc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wdc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q72IT0_THET2 Q72IT0_THET2] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/2wdc_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wdc ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
SoxB is an essential component of the bacterial Sox sulfur oxidation pathway. SoxB contains a di-manganese(II) site and is proposed to catalyze the release of sulfate from a protein-bound cysteine S-thiosulfonate. A direct assay for SoxB activity is described. The structure of recombinant Thermus thermophilus SoxB was determined by x-ray crystallography to a resolution of 1.5 A. Structures were also determined for SoxB in complex with the substrate analogue thiosulfate and in complex with the product sulfate. A mechanistic model for SoxB is proposed based on these structures. | |||
Mechanism for the hydrolysis of a sulfur-sulfur bond based on the crystal structure of the thiosulfohydrolase SoxB.,Sauve V, Roversi P, Leath KJ, Garman EF, Antrobus R, Lea SM, Berks BC J Biol Chem. 2009 Aug 7;284(32):21707-18. Epub 2009 Jun 16. PMID:19535341<ref>PMID:19535341</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2wdc" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
[[Category: Thermus thermophilus]] | <references/> | ||
[[Category: Antrobus | __TOC__ | ||
[[Category: Berks | </StructureSection> | ||
[[Category: Garman | [[Category: Large Structures]] | ||
[[Category: Lea | [[Category: Thermus thermophilus HB27]] | ||
[[Category: Leath | [[Category: Antrobus R]] | ||
[[Category: Roversi | [[Category: Berks BC]] | ||
[[Category: Sauve | [[Category: Garman EF]] | ||
[[Category: Lea SM]] | |||
[[Category: Leath KJ]] | |||
[[Category: Roversi P]] | |||
[[Category: Sauve V]] | |||
Latest revision as of 13:11, 9 May 2024
Termus thermophilus Sulfate thiohydrolase SoxB in complex with glycerolTermus thermophilus Sulfate thiohydrolase SoxB in complex with glycerol
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSoxB is an essential component of the bacterial Sox sulfur oxidation pathway. SoxB contains a di-manganese(II) site and is proposed to catalyze the release of sulfate from a protein-bound cysteine S-thiosulfonate. A direct assay for SoxB activity is described. The structure of recombinant Thermus thermophilus SoxB was determined by x-ray crystallography to a resolution of 1.5 A. Structures were also determined for SoxB in complex with the substrate analogue thiosulfate and in complex with the product sulfate. A mechanistic model for SoxB is proposed based on these structures. Mechanism for the hydrolysis of a sulfur-sulfur bond based on the crystal structure of the thiosulfohydrolase SoxB.,Sauve V, Roversi P, Leath KJ, Garman EF, Antrobus R, Lea SM, Berks BC J Biol Chem. 2009 Aug 7;284(32):21707-18. Epub 2009 Jun 16. PMID:19535341[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||