2w1a: Difference between revisions

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-[[Image:2w1a.png|left|200px]]
-<!--
+==Non-covalent complex between dahp synthase and chorismate mutase from Mycobacterium tuberculosis with bound tsa==
-The line below this paragraph, containing "STRUCTURE_2w1a", creates the "Structure Box" on the page.
+<StructureSection load='2w1a' size='340' side='right'caption='[[2w1a]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2w1a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W1A FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CE1:O-DODECANYL+OCTAETHYLENE+GLYCOL'>CE1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TSA:8-HYDROXY-2-OXA-BICYCLO[3.3.1]NON-6-ENE-3,5-DICARBOXYLIC+ACID'>TSA</scene></td></tr>
-{{STRUCTURE_2w1a|  PDB=2w1a  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w1a OCA], [https://pdbe.org/2w1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w1a RCSB], [https://www.ebi.ac.uk/pdbsum/2w1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w1a ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AROG_MYCTU AROG_MYCTU] Catalyzes an aldol-like condensation reaction between phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate (E4P) to generate 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P) and inorganic phosphate.<ref>PMID:16288916</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w1/2w1a_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w1a ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Chorismate mutase catalyzes a key step in the shikimate biosynthetic pathway towards phenylalanine and tyrosine. Curiously, the intracellular chorismate mutase of Mycobacterium tuberculosis (MtCM; Rv0948c) has poor activity and lacks prominent active-site residues. However, its catalytic efficiency increases &gt;100-fold on addition of DAHP synthase (MtDS; Rv2178c), another shikimate-pathway enzyme. The 2.35 A crystal structure of the MtCM-MtDS complex bound to a transition-state analogue shows a central core formed by four MtDS subunits sandwiched between two MtCM dimers. Structural comparisons imply catalytic activation to be a consequence of the repositioning of MtCM active-site residues on binding to MtDS. The mutagenesis of the C-terminal extrusion of MtCM establishes conserved residues as part of the activation machinery. The chorismate-mutase activity of the complex, but not of MtCM alone, is inhibited synergistically by phenylalanine and tyrosine. The complex formation thus endows the shikimate pathway of M. tuberculosis with an important regulatory feature. Experimental evidence suggests that such non-covalent enzyme complexes comprising an AroQ(delta) subclass chorismate mutase like MtCM are abundant in the bacterial order Actinomycetales.
-===Non-covalent complex between dahp synthase and chorismate mutase from Mycobacterium tuberculosis with bound tsa===
+Structure and function of a complex between chorismate mutase and DAHP synthase: efficiency boost for the junior partner.,Sasso S, Okvist M, Roderer K, Gamper M, Codoni G, Krengel U, Kast P EMBO J. 2009 Jul 22;28(14):2128-42. Epub 2009 Jun 25. PMID:19556970<ref>PMID:19556970</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2w1a" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_19556970}}, adds the Publication Abstract to the page
+*[[DAHP synthase 3D structures|DAHP synthase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 19556970 is the PubMed ID number.
+*[[3D structures of chorismate mutase|3D structures of chorismate mutase]]
--->
+== References ==
-{{ABSTRACT_PUBMED_19556970}}
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-[[2w1a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W1A OCA].
+[[Category: Large Structures]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-==Reference==
+[[Category: Codoni G]]
-<ref group="xtra">PMID:019556970</ref><references group="xtra"/>
+[[Category: Gamper M]]
-[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
+[[Category: Kast P]]
-[[Category: Mycobacterium tuberculosis]]
+[[Category: Krengel U]]
-[[Category: Codoni, G.]]
+[[Category: Okvist M]]
-[[Category: Gamper, M.]]
+[[Category: Roderer K]]
-[[Category: Kast, P.]]
+[[Category: Sasso S]]
-[[Category: Krengel, U.]]
-[[Category: Okvist, M.]]
-[[Category: Roderer, K.]]
-[[Category: Sasso, S.]]
-[[Category: Aromatic amino acid biosynthesis]]
-[[Category: Multi-enzyme complex]]
-[[Category: Protein-protein interaction]]
-[[Category: Shikimate pathway]]
-[[Category: Transferase-isomerase complex]]