2jz4: Difference between revisions
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== | ==Putative 32 kDa myrosinase binding protein At3g16450.1 from Arabidopsis thaliana== | ||
[[2jz4]] is a 1 chain structure with sequence from [ | <StructureSection load='2jz4' size='340' side='right'caption='[[2jz4]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2jz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JZ4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jz4 OCA], [https://pdbe.org/2jz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jz4 RCSB], [https://www.ebi.ac.uk/pdbsum/2jz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jz4 ProSAT], [https://www.topsan.org/Proteins/CESG/2jz4 TOPSAN]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/JAL33_ARATH JAL33_ARATH] Sugar-binding protein showing significant affinity for (Glc alpha(1-4)Glc)(3) maltohexaose, (Glc alpha(1-6)Glc)(3) isomaltohexaose, Gal alpha(1-4)Gal beta(1-4)Glc, GalNAc alpha(1-3)(Fuc alpha(1-2)) and Gal beta(1-3)(Fuc alpha(1-4))GlcNAc beta(1-3)Gal beta(1-4)Glc.<ref>PMID:19021763</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/2jz4_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jz4 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The product of gene At3g16450.1 from Arabidopsis thaliana is a 32 kDa, 299-residue protein classified as resembling a myrosinase-binding protein (MyroBP). MyroBPs are found in plants as part of a complex with the glucosinolate-degrading enzyme myrosinase, and are suspected to play a role in myrosinase-dependent defense against pathogens. Many MyroBPs and MyroBP-related proteins are composed of repeated homologous sequences with unknown structure. We report here the three-dimensional structure of the At3g16450.1 protein from Arabidopsis, which consists of two tandem repeats. Because the size of the protein is larger than that amenable to high-throughput analysis by uniform (13)C/(15)N labeling methods, we used stereo-array isotope labeling (SAIL) technology to prepare an optimally (2)H/(13)C/(15)N-labeled sample. NMR data sets collected using the SAIL protein enabled us to assign (1)H, (13)C and (15)N chemical shifts to 95.5% of all atoms, even at a low concentration (0.2 mm) of protein product. We collected additional NOESY data and determined the three-dimensional structure using the cyana software package. The structure, the first for a MyroBP family member, revealed that the At3g16450.1 protein consists of two independent but similar lectin-fold domains, each composed of three beta-sheets. | |||
Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR.,Takeda M, Sugimori N, Torizawa T, Terauchi T, Ono AM, Yagi H, Yamaguchi Y, Kato K, Ikeya T, Jee J, Guntert P, Aceti DJ, Markley JL, Kainosho M FEBS J. 2008 Dec;275(23):5873-84. PMID:19021763<ref>PMID:19021763</ref> | |||
<ref | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2jz4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Aceti DJ]] | ||
[[Category: Guntert | [[Category: Guntert P]] | ||
[[Category: Ikeya | [[Category: Ikeya T]] | ||
[[Category: Kainosho | [[Category: Kainosho M]] | ||
[[Category: Kato | [[Category: Kato K]] | ||
[[Category: Markley | [[Category: Markley JL]] | ||
[[Category: Ono | [[Category: Ono AM]] | ||
[[Category: Sugimori | [[Category: Sugimori N]] | ||
[[Category: Takeda | [[Category: Takeda N]] | ||
[[Category: Terauchi | [[Category: Terauchi T]] | ||
[[Category: Torizawa | [[Category: Torizawa T]] | ||
[[Category: Yagi | [[Category: Yagi H]] | ||
[[Category: Yamaguchi | [[Category: Yamaguchi Y]] | ||