2j7n: Difference between revisions

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-{{Seed}}
-[[Image:2j7n.png|left|200px]]
-<!--
+==Structure of the RNAi polymerase from Neurospora crassa==
-The line below this paragraph, containing "STRUCTURE_2j7n", creates the "Structure Box" on the page.
+<StructureSection load='2j7n' size='340' side='right'caption='[[2j7n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2j7n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J7N FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-{{STRUCTURE_2j7n|  PDB=2j7n  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7n OCA], [https://pdbe.org/2j7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j7n RCSB], [https://www.ebi.ac.uk/pdbsum/2j7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7n ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9Y7G6_NEUCS Q9Y7G6_NEUCS]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/2j7n_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j7n ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+RNA silencing refers to a group of RNA-induced gene-silencing mechanisms that developed early in the eukaryotic lineage, probably for defence against pathogens and regulation of gene expression. In plants, protozoa, fungi, and nematodes, but apparently not insects and vertebrates, it involves a cell-encoded RNA-dependent RNA polymerase (cRdRP) that produces double-stranded RNA triggers from aberrant single-stranded RNA. We report the 2.3-A resolution crystal structure of QDE-1, a cRdRP from Neurospora crassa, and find that it forms a relatively compact dimeric molecule, each subunit of which comprises several domains with, at its core, a catalytic apparatus and protein fold strikingly similar to the catalytic core of the DNA-dependent RNA polymerases responsible for transcription. This evolutionary link between the two enzyme types suggests that aspects of RNA silencing in some organisms may recapitulate transcription/replication pathways functioning in the ancient RNA-based world.
-===STRUCTURE OF THE RNAI POLYMERASE FROM NEUROSPORA CRASSA===
+The structure of an RNAi polymerase links RNA silencing and transcription.,Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM PLoS Biol. 2006 Dec;4(12):e434. PMID:17147473<ref>PMID:17147473</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2j7n" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_17147473}}, adds the Publication Abstract to the page
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 17147473 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17147473}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-J7N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7N OCA].
-==Reference==
-The structure of an RNAi polymerase links RNA silencing and transcription., Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM, PLoS Biol. 2006 Dec;4(12):e434. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17147473 17147473]
 [[Category: Neurospora crassa]]
-[[Category: Single protein]]
+[[Category: Bamford DH]]
-[[Category: Bamford, D H.]]
+[[Category: Grimes JM]]
-[[Category: Grimes, J M.]]
+[[Category: Koivunen MRL]]
-[[Category: Koivunen, M R.L.]]
+[[Category: Makeyev EV]]
-[[Category: Makeyev, E V.]]
+[[Category: Salgado PS]]
-[[Category: Salgado, P S.]]
+[[Category: Stuart DI]]
-[[Category: Stuart, D I.]]
-[[Category: Hydrolase]]
-[[Category: Hypothetical protein]]
-[[Category: Rna dependent rna polymerase]]
-[[Category: Rna-directed rna polymerase]]
-[[Category: Rnai response]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:49:01 2008''