2j6g: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:2j6g.png|left|200px]]
-<!--
+==FaeG from F4ac ETEC strain 5_95, produced in tobacco plant chloroplast==
-The line below this paragraph, containing "STRUCTURE_2j6g", creates the "Structure Box" on the page.
+<StructureSection load='2j6g' size='340' side='right'caption='[[2j6g]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2j6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J6G FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
-{{STRUCTURE_2j6g|  PDB=2j6g  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j6g OCA], [https://pdbe.org/2j6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j6g RCSB], [https://www.ebi.ac.uk/pdbsum/2j6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j6g ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q6T3W5_ECOLX Q6T3W5_ECOLX]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j6/2j6g_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j6g ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+F4 fimbriae encoded by the fae operon are the major colonization factors associated with porcine neonatal and postweaning diarrhoea caused by enterotoxigenic Escherichia coli (ETEC). Via the chaperone/usher pathway, the F4 fimbriae are assembled as long polymers of the major subunit FaeG, which also possesses the adhesive properties of the fimbriae. Intrinsically, the incomplete fold of fimbrial subunits renders them unstable and susceptible to aggregation and/or proteolytic degradation in the absence of a specific periplasmic chaperone. In order to test the possibility of producing FaeG in plants, FaeG expression was studied in transgenic tobacco plants. FaeG was directed to different subcellular compartments by specific targeting signals. Targeting of FaeG to the chloroplast results in much higher yields than FaeG targeting to the endoplasmic reticulum or the apoplast. Two chloroplast-targeted FaeG variants were purified from tobacco plants and crystallized. The crystal structures show that chloroplasts circumvent the absence of the fimbrial assembly machinery by assembling FaeG into strand-swapped dimers. Furthermore, the structures reveal how FaeG combines the structural requirements of a major fimbrial subunit with its adhesive role by grafting an additional domain on its Ig-like core.
-===FAEG FROM F4AC ETEC STRAIN 5_95, PRODUCED IN TOBACCO PLANT CHLOROPLAST===
+Chloroplasts assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers.,Van Molle I, Joensuu JJ, Buts L, Panjikar S, Kotiaho M, Bouckaert J, Wyns L, Niklander-Teeri V, De Greve H J Mol Biol. 2007 May 4;368(3):791-9. Epub 2007 Feb 22. PMID:17368480<ref>PMID:17368480</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_17368480}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2j6g" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 17368480 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17368480}}
+__TOC__
+</StructureSection>
-==About this Structure==
-J6G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6G OCA].
-==Reference==
-Chloroplasts assemble the major subunit FaeG of Escherichia coli F4 (K88) fimbriae to strand-swapped dimers., Van Molle I, Joensuu JJ, Buts L, Panjikar S, Kotiaho M, Bouckaert J, Wyns L, Niklander-Teeri V, De Greve H, J Mol Biol. 2007 May 4;368(3):791-9. Epub 2007 Feb 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17368480 17368480]
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bouckaert, J.]]
+[[Category: Bouckaert J]]
-[[Category: Buts, L.]]
+[[Category: Buts L]]
-[[Category: Greve, H De.]]
+[[Category: De Greve H]]
-[[Category: Joensuu, J J.]]
+[[Category: Joensuu JJ]]
-[[Category: Kotiaho, M.]]
+[[Category: Kotiaho M]]
-[[Category: Molle, I Van.]]
+[[Category: Niklander-Teeri V]]
-[[Category: Niklander-Teeri, V.]]
+[[Category: Panjikar S]]
-[[Category: Panjikar, S.]]
+[[Category: Van Molle I]]
-[[Category: Wyns, L.]]
+[[Category: Wyns L]]
-[[Category: Cell adhesion]]
-[[Category: Chaperone-usher pathway]]
-[[Category: Chloroplast targeting]]
-[[Category: F4 fimbriae]]
-[[Category: Ig-fold]]
-[[Category: Strand swapping]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:02:02 2008''